TY - JOUR
T1 - Developing SNPs and STR DNA markers for snub-nosed monkeys (Rhinopithecus roxellana) using next-generation sequencing technology
AU - Yan, Caie
AU - Shattuck, Milena R.
AU - Thimmapuram, Jyothi
AU - Vullaganti, Deepika
AU - Malhi, Ripan
N1 - Funding Information:
Acknowledgments This project was supported by a National Science Foundation Dissertation Improvement Grant (BCS-0824466).
PY - 2012/6
Y1 - 2012/6
N2 - In this study, we describe a protocol of generating a large number of candidate SNPs and STR loci quickly and cost-efficiently for an endangered species, snub-nosed monkeys (Rhinopithecus roxellana), using next-generation sequencing technology. First, we built a reduced representation library of snub-nosed monkeys through digesting the DNA of four individuals using HaeIII and pooling the 350-500 bp fragments together. Second, we sequenced the DNA pool using pyrosequencing technology. Third, we analyzed sequencing reads using bioinformatic software. We detected 23 putative SNPs, 10 polymorphic STR loci, and 347 candidate STR loci (number of repeats greater than 9). The markers generated by this study can be used to address questions regarding genetic diversity, population structure, phylogeography, and conservation of this endangered primate.
AB - In this study, we describe a protocol of generating a large number of candidate SNPs and STR loci quickly and cost-efficiently for an endangered species, snub-nosed monkeys (Rhinopithecus roxellana), using next-generation sequencing technology. First, we built a reduced representation library of snub-nosed monkeys through digesting the DNA of four individuals using HaeIII and pooling the 350-500 bp fragments together. Second, we sequenced the DNA pool using pyrosequencing technology. Third, we analyzed sequencing reads using bioinformatic software. We detected 23 putative SNPs, 10 polymorphic STR loci, and 347 candidate STR loci (number of repeats greater than 9). The markers generated by this study can be used to address questions regarding genetic diversity, population structure, phylogeography, and conservation of this endangered primate.
KW - Pyrosequencing
KW - Rhinopithecus roxellana
KW - SNP
KW - STR
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U2 - 10.1007/s12686-011-9572-7
DO - 10.1007/s12686-011-9572-7
M3 - Article
AN - SCOPUS:84892870765
SN - 1877-7252
VL - 4
SP - 451
EP - 453
JO - Conservation Genetics Resources
JF - Conservation Genetics Resources
IS - 2
ER -