Detoxification of corn stover hydrolysate using surfactant-based aqueous two phase system

Pradip B. Dhamole, Bin Wang, Hao Feng

Research output: Contribution to journalArticlepeer-review


BACKGROUND: Pretreatment methods used to break down lignocellulose structure lead to the generation of compounds that inhibit ethanol fermentation. Hence, removal of these compounds is essential for improved fermentability. The surfactant-based cloud point extraction (CPE) aqueous two phase system is a new method having the potential for separation and recovery of inhibitors. The present work examines the potential of surfactants-based CPE systems as a detoxification method. RESULTS: Surfactants L62D and L62LF achieved more than 90% removal of phenolic compounds and less than 20% removal of acetic acid and HMF from a model system when the surfactant concentration was 1% and 5%. With untreated simulated hydrolysate containing inhibitors the fermentation was inhibited completely. Detoxification of the same hydrolysate with L62D (1% and 5%) and L62LF (1% and 5%) showed complete fermentation with high ethanol productivity (0.61gL-1h-1). Similarly, the detoxified corn stover hydrolysate showed delayed fermentation. The productivity increased by 30% after detoxification with 1% of the two surfactants and by 100% with 5% of the surfactants (L62D and L62LF). CONCLUSION: The new surfactant-based detoxification method significantly improved the fermentability of simulated hydrolysate and corn stover hydrolysate.

Original languageEnglish (US)
Pages (from-to)1744-1749
Number of pages6
JournalJournal of Chemical Technology and Biotechnology
Issue number9
StatePublished - Sep 1 2013


  • Corn stover
  • Detoxification
  • Ethanol
  • Surfactant

ASJC Scopus subject areas

  • Biotechnology
  • Chemical Engineering(all)
  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Waste Management and Disposal
  • Pollution
  • Organic Chemistry
  • Inorganic Chemistry

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