Values are the fundamental reasons why people engage in conservation behaviors. Recent research has called for a more refined approach to studying values in a way that accounts for the concept of eudaimonia. However, the empirical properties for a eudaimonic value scale have not been tested given that previous investigations have remained at the theoretical level. Drawing from an on-site survey of visitors to Denali National Park and Preserve, Alaska, we used a latent profile analysis used a latent profile analysis to better understand the expression of multiple values of nature. Specifically, we segmented respondents by their value orientations with a particular focus on evaluating eudaimonic and hedonic values, alongside the established dimensions of altruistic, biospheric, and egoistic values. We identified 4 distinct subgroups defined by value orientations and validated these subgroups based on measures of conservation behavior and sociodemographic characteristics (e.g., age). These results indicated campaign messaging should harness a combination of eudaimonic, biospheric, and altruistic values to propel individual behavior. We also observed that hedonic and egoistic values defined how people related to nature and played a role, albeit less pronounced, in motivating them to take action. Our study is one of the first efforts to operationalize eudaimonia in a conservation context; thus, we have opened a new avenue for protected-area managers to align their strategies with the underlying values of stakeholders.