Determining the efficacy of the bovine amniotic membrane homogenate during the healing process in rabbits' ex vivo corneas

Erotides Capistrano da Silva, Mariano Carossino, Kathryn M. Smith-Fleming, Ingeborg M. Langohr, Bianca da C. Martins

Research output: Contribution to journalArticlepeer-review


Objective: To evaluate the efficacy of bovine amniotic membrane homogenate (BAMH) on wounded ex vivo rabbit corneas. Procedure: Eighteen corneas obtained from normal rabbit eyes were wounded equally using a 6 mm trephine and cultured into an air-liquid interface model. Corneas were treated with phosphate-buffered saline (PBS) (n = 6, control group), 0.2% ethylenediaminetetraacetic acid (EDTA; n = 6), or BAMH (n = 6). All treatments were applied topically 6 times/day. Each cornea was macrophotographed daily with and without fluorescein stain to assess epithelialization and haziness. After 7 days, corneal transparency was evaluated, and the tissues prepared for histologic analysis of viability, and total and epithelial thickness. Results: The mean epithelialization time was 6.2 ± 0.82 days for the control group, 6.2 ± 0.75 days for the EDTA-treated group, and 5.1 ± 0.40 days for the BAMH-treated group, demonstrating a significant difference between the BAMH and the other groups. The corneas that received EDTA had better transparency compared with the other groups. Histologically, all corneas had adequate morphology and architecture after healing. Analysis of corneal and epithelial thickness revealed no significant difference among groups. Conclusion: Bovine amniotic membrane homogenate is an effective and promising treatment for stromal and epithelial ulcers.

Original languageEnglish (US)
Pages (from-to)380-390
Number of pages11
JournalVeterinary Ophthalmology
Issue number4
StatePublished - Jul 2021


  • bovine amniotic membrane homogenate
  • cornea
  • ex vivo
  • rabbits
  • wound healing

ASJC Scopus subject areas

  • veterinary(all)


Dive into the research topics of 'Determining the efficacy of the bovine amniotic membrane homogenate during the healing process in rabbits' ex vivo corneas'. Together they form a unique fingerprint.

Cite this