TY - JOUR
T1 - Determination of thiafentanil in plasma using LC-MS
AU - Cox, Sherry
AU - Bergman, Joan
AU - Allender, Matthew C.
AU - Beckmen, Kimberlee
AU - Lance, William
N1 - Publisher Copyright:
© The Author(s) 2019. Published by Oxford University Press. All rights reserved. For Permissions, please email: [email protected]
PY - 2020/1/1
Y1 - 2020/1/1
N2 - A new method of analysis has been developed and validated for the determination of thiafentanil in plasma. After protein precipitation, samples were separated on an XBridge BEH C18 column and quantified using mass spectrometry. The mobile phase was a mixture of water with 0.1% formic acid and acetonitrile with 0.1% formic acid (90:10). The standard curve ranged from 0.1 to 25 ng/mL. Intra- and Inter-assay variability for thiafentanil was less than 10%, and the average recovery was greater than 95%. The lower limit of quantification was 0.1 ng/mL. This is the first validated method for thiafentanil analysis in plasma.
AB - A new method of analysis has been developed and validated for the determination of thiafentanil in plasma. After protein precipitation, samples were separated on an XBridge BEH C18 column and quantified using mass spectrometry. The mobile phase was a mixture of water with 0.1% formic acid and acetonitrile with 0.1% formic acid (90:10). The standard curve ranged from 0.1 to 25 ng/mL. Intra- and Inter-assay variability for thiafentanil was less than 10%, and the average recovery was greater than 95%. The lower limit of quantification was 0.1 ng/mL. This is the first validated method for thiafentanil analysis in plasma.
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U2 - 10.1093/chromsci/bmz098
DO - 10.1093/chromsci/bmz098
M3 - Article
C2 - 31813994
AN - SCOPUS:85083002911
SN - 0021-9665
VL - 58
SP - 1
EP - 4
JO - Journal of Chromatographic Science
JF - Journal of Chromatographic Science
IS - 1
ER -