Determination of metronidazole in adult artemia using high performance liquid chromatography

Sherry Cox, Matthew C. Allender, Jason Yarbrough

Research output: Contribution to journalArticlepeer-review


A new HPLC procedure for the determination of metronidazole, a nitroimidazole, in adult Artemia (live brine shrimp) has been developed and validated. Following a liquid extraction using methanol, samples were separated by isocratic reversed-phase HPLC on a Symmetry C18 column and quantified using UV detection at 324nm. The mobile phase was a mixture of water and methanol, with a flow-rate of 1.0ml/min. The procedure produced a linear curve over the concentration range 1-500μg/gm with a lower limit of quantification of 0.25μg/gm. Intra and inter-assay variability was less than 10%. The development of the assay allowed the determination of metronidazole in a live brine shrimp feeding study.

Original languageEnglish (US)
Pages (from-to)89-96
Number of pages8
JournalJournal of Liquid Chromatography and Related Technologies
Issue number1
StatePublished - Jan 2010
Externally publishedYes


  • Antimicrobial
  • Aquaculture
  • Artemia
  • Bioencapsulation
  • HPLC
  • Metronidazole

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Pharmaceutical Science
  • Clinical Biochemistry


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