Determination of metronidazole in adult artemia using high performance liquid chromatography

Sherry Cox; Matthew C. Allender; Jason Yarbrough

doi:10.1080/10826070903430381

Determination of metronidazole in adult artemia using high performance liquid chromatography

Sherry Cox, Matthew C. Allender, Jason Yarbrough

Research output: Contribution to journal › Article › peer-review

Abstract

A new HPLC procedure for the determination of metronidazole, a nitroimidazole, in adult Artemia (live brine shrimp) has been developed and validated. Following a liquid extraction using methanol, samples were separated by isocratic reversed-phase HPLC on a Symmetry C₁₈ column and quantified using UV detection at 324nm. The mobile phase was a mixture of water and methanol, with a flow-rate of 1.0ml/min. The procedure produced a linear curve over the concentration range 1-500μg/gm with a lower limit of quantification of 0.25μg/gm. Intra and inter-assay variability was less than 10%. The development of the assay allowed the determination of metronidazole in a live brine shrimp feeding study.

Original language	English (US)
Pages (from-to)	89-96
Number of pages	8
Journal	Journal of Liquid Chromatography and Related Technologies
Volume	33
Issue number	1
DOIs	https://doi.org/10.1080/10826070903430381
State	Published - Jan 2010
Externally published	Yes

Keywords

Antimicrobial
Aquaculture
Artemia
Bioencapsulation
HPLC
Metronidazole

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Pharmaceutical Science
Clinical Biochemistry

Online availability

10.1080/10826070903430381

Library availability

Discover UIUC Full Text

Cite this

@article{ddfda1bfb9014d309aee322c9f30ad85,

title = "Determination of metronidazole in adult artemia using high performance liquid chromatography",

abstract = "A new HPLC procedure for the determination of metronidazole, a nitroimidazole, in adult Artemia (live brine shrimp) has been developed and validated. Following a liquid extraction using methanol, samples were separated by isocratic reversed-phase HPLC on a Symmetry C18 column and quantified using UV detection at 324nm. The mobile phase was a mixture of water and methanol, with a flow-rate of 1.0ml/min. The procedure produced a linear curve over the concentration range 1-500μg/gm with a lower limit of quantification of 0.25μg/gm. Intra and inter-assay variability was less than 10%. The development of the assay allowed the determination of metronidazole in a live brine shrimp feeding study.",

keywords = "Antimicrobial, Aquaculture, Artemia, Bioencapsulation, HPLC, Metronidazole",

author = "Sherry Cox and Allender, {Matthew C.} and Jason Yarbrough",

year = "2010",

month = jan,

doi = "10.1080/10826070903430381",

language = "English (US)",

volume = "33",

pages = "89--96",

journal = "Journal of Liquid Chromatography and Related Technologies",

issn = "1082-6076",

publisher = "Taylor and Francis Ltd.",

number = "1",

}

TY - JOUR

T1 - Determination of metronidazole in adult artemia using high performance liquid chromatography

AU - Cox, Sherry

AU - Allender, Matthew C.

AU - Yarbrough, Jason

PY - 2010/1

Y1 - 2010/1

N2 - A new HPLC procedure for the determination of metronidazole, a nitroimidazole, in adult Artemia (live brine shrimp) has been developed and validated. Following a liquid extraction using methanol, samples were separated by isocratic reversed-phase HPLC on a Symmetry C18 column and quantified using UV detection at 324nm. The mobile phase was a mixture of water and methanol, with a flow-rate of 1.0ml/min. The procedure produced a linear curve over the concentration range 1-500μg/gm with a lower limit of quantification of 0.25μg/gm. Intra and inter-assay variability was less than 10%. The development of the assay allowed the determination of metronidazole in a live brine shrimp feeding study.

AB - A new HPLC procedure for the determination of metronidazole, a nitroimidazole, in adult Artemia (live brine shrimp) has been developed and validated. Following a liquid extraction using methanol, samples were separated by isocratic reversed-phase HPLC on a Symmetry C18 column and quantified using UV detection at 324nm. The mobile phase was a mixture of water and methanol, with a flow-rate of 1.0ml/min. The procedure produced a linear curve over the concentration range 1-500μg/gm with a lower limit of quantification of 0.25μg/gm. Intra and inter-assay variability was less than 10%. The development of the assay allowed the determination of metronidazole in a live brine shrimp feeding study.

KW - Antimicrobial

KW - Aquaculture

KW - Artemia

KW - Bioencapsulation

KW - HPLC

KW - Metronidazole

UR - http://www.scopus.com/inward/record.url?scp=73849103883&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=73849103883&partnerID=8YFLogxK

U2 - 10.1080/10826070903430381

DO - 10.1080/10826070903430381

M3 - Article

AN - SCOPUS:73849103883

SN - 1082-6076

VL - 33

SP - 89

EP - 96

JO - Journal of Liquid Chromatography and Related Technologies

JF - Journal of Liquid Chromatography and Related Technologies

IS - 1

ER -

Determination of metronidazole in adult artemia using high performance liquid chromatography

Abstract

Keywords

ASJC Scopus subject areas

Online availability

Library availability

Related links

Fingerprint

Cite this