Determination of magnesium binding to macromolecules

Dean L. Olson, David W. Deerfield, Pola Berkowitz, Richard G. Hiskey, Lee G. Pedersen

Research output: Contribution to journalArticlepeer-review


An equilibrium dialysis technique for examining magnesium binding to macromolecules is described. The technique is used to determine the binding constants of magnesium to human prothrombin. This procedure should be of great utility for many biochemical systems which exhibit magnesium affinity.

Original languageEnglish (US)
Pages (from-to)468-470
Number of pages3
JournalAnalytical Biochemistry
Issue number2
StatePublished - Feb 1 1987
Externally publishedYes


  • coagulation
  • equilibrium dialysis
  • magnesium binding
  • prothrombin
  • γ-carboxyglutamic acid

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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