Determination of magnesium binding to macromolecules

Dean L. Olson; David W. Deerfield; Pola Berkowitz; Richard G. Hiskey; Lee G. Pedersen

doi:10.1016/0003-2697(87)90077-7

Determination of magnesium binding to macromolecules

Dean L. Olson, David W. Deerfield, Pola Berkowitz, Richard G. Hiskey, Lee G. Pedersen

Research output: Contribution to journal › Article › peer-review

Abstract

An equilibrium dialysis technique for examining magnesium binding to macromolecules is described. The technique is used to determine the binding constants of magnesium to human prothrombin. This procedure should be of great utility for many biochemical systems which exhibit magnesium affinity.

Original language	English (US)
Pages (from-to)	468-470
Number of pages	3
Journal	Analytical Biochemistry
Volume	160
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(87)90077-7
State	Published - Feb 1 1987
Externally published	Yes

Keywords

coagulation
equilibrium dialysis
magnesium binding
prothrombin
γ-carboxyglutamic acid

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Online availability

10.1016/0003-2697(87)90077-7

Library availability

Discover UIUC Full Text

Cite this

@article{c72333a4ce7b4b2abee216596d86dad6,

title = "Determination of magnesium binding to macromolecules",

abstract = "An equilibrium dialysis technique for examining magnesium binding to macromolecules is described. The technique is used to determine the binding constants of magnesium to human prothrombin. This procedure should be of great utility for many biochemical systems which exhibit magnesium affinity.",

keywords = "coagulation, equilibrium dialysis, magnesium binding, prothrombin, γ-carboxyglutamic acid",

author = "Olson, {Dean L.} and Deerfield, {David W.} and Pola Berkowitz and Hiskey, {Richard G.} and Pedersen, {Lee G.}",

note = "Funding Information: {\textquoteright} This work was supported by Grants HL-27995 (L.G.P.) and HL-20161 (R.G.H.) from the National Institutes of Health, U.S. Public Health Service. * To whom correspondence should be addressed.",

year = "1987",

month = feb,

day = "1",

doi = "10.1016/0003-2697(87)90077-7",

language = "English (US)",

volume = "160",

pages = "468--470",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Determination of magnesium binding to macromolecules

AU - Olson, Dean L.

AU - Deerfield, David W.

AU - Berkowitz, Pola

AU - Hiskey, Richard G.

AU - Pedersen, Lee G.

N1 - Funding Information: ’ This work was supported by Grants HL-27995 (L.G.P.) and HL-20161 (R.G.H.) from the National Institutes of Health, U.S. Public Health Service. * To whom correspondence should be addressed.

PY - 1987/2/1

Y1 - 1987/2/1

N2 - An equilibrium dialysis technique for examining magnesium binding to macromolecules is described. The technique is used to determine the binding constants of magnesium to human prothrombin. This procedure should be of great utility for many biochemical systems which exhibit magnesium affinity.

AB - An equilibrium dialysis technique for examining magnesium binding to macromolecules is described. The technique is used to determine the binding constants of magnesium to human prothrombin. This procedure should be of great utility for many biochemical systems which exhibit magnesium affinity.

KW - coagulation

KW - equilibrium dialysis

KW - magnesium binding

KW - prothrombin

KW - γ-carboxyglutamic acid

UR - http://www.scopus.com/inward/record.url?scp=0023135971&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023135971&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(87)90077-7

DO - 10.1016/0003-2697(87)90077-7

M3 - Article

C2 - 3578772

AN - SCOPUS:0023135971

SN - 0003-2697

VL - 160

SP - 468

EP - 470

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

Determination of magnesium binding to macromolecules

Abstract

Keywords

ASJC Scopus subject areas

Online availability

Library availability

Related links

Fingerprint

Cite this