Abstract
A new HPLC procedure for the determination of fenbendazole, a benzimidazole, in adult Artemia (live brine shrimp) has been developed and validated. Following a liquid extraction using ethyl acetate, samples were separated by reversed-phase HPLC on a Sunfire C 18 column and quantified using UV detection at 215nm. The mobile phase was a mixture of 37.5mM potassium dihydrogen phosphate pH 3.75 and acetonitrile, with a flow-rate of 0.9mL/min. The procedure produced a linear curve over the concentration range of 100-5000μg/gm with a lower limit of quantification of 1 μg/gm. Intra- and inter-assay variability was less than 8%. The development of the assay allowed the determination of fenbendazole in a live brine shrimp feeding study.
Original language | English (US) |
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Pages (from-to) | 1536-1545 |
Number of pages | 10 |
Journal | Journal of Liquid Chromatography and Related Technologies |
Volume | 34 |
Issue number | 15 |
DOIs | |
State | Published - Sep 1 2011 |
Externally published | Yes |
Keywords
- HPLC
- anthelmintics
- aquaculture
- artemia
- bioencapsulation
- fenbendazole
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Pharmaceutical Science
- Clinical Biochemistry