Determination of fenbendazole in adult Artemia using reverse-phase liquid chromatography

Sherry Cox, Matthew C. Allender, Jason Yarbrough

Research output: Contribution to journalArticle

Abstract

A new HPLC procedure for the determination of fenbendazole, a benzimidazole, in adult Artemia (live brine shrimp) has been developed and validated. Following a liquid extraction using ethyl acetate, samples were separated by reversed-phase HPLC on a Sunfire C 18 column and quantified using UV detection at 215nm. The mobile phase was a mixture of 37.5mM potassium dihydrogen phosphate pH 3.75 and acetonitrile, with a flow-rate of 0.9mL/min. The procedure produced a linear curve over the concentration range of 100-5000μg/gm with a lower limit of quantification of 1 μg/gm. Intra- and inter-assay variability was less than 8%. The development of the assay allowed the determination of fenbendazole in a live brine shrimp feeding study.

Original languageEnglish (US)
Pages (from-to)1536-1545
Number of pages10
JournalJournal of Liquid Chromatography and Related Technologies
Volume34
Issue number15
DOIs
StatePublished - Sep 1 2011
Externally publishedYes

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Keywords

  • HPLC
  • anthelmintics
  • aquaculture
  • artemia
  • bioencapsulation
  • fenbendazole

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Pharmaceutical Science
  • Clinical Biochemistry

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