Determination of fenbendazole in adult Artemia using reverse-phase liquid chromatography

Sherry Cox; Matthew C. Allender; Jason Yarbrough

doi:10.1080/10826076.2011.575977

Determination of fenbendazole in adult Artemia using reverse-phase liquid chromatography

Sherry Cox, Matthew C. Allender, Jason Yarbrough

Research output: Contribution to journal › Article › peer-review

Abstract

A new HPLC procedure for the determination of fenbendazole, a benzimidazole, in adult Artemia (live brine shrimp) has been developed and validated. Following a liquid extraction using ethyl acetate, samples were separated by reversed-phase HPLC on a Sunfire C ₁₈ column and quantified using UV detection at 215nm. The mobile phase was a mixture of 37.5mM potassium dihydrogen phosphate pH 3.75 and acetonitrile, with a flow-rate of 0.9mL/min. The procedure produced a linear curve over the concentration range of 100-5000μg/gm with a lower limit of quantification of 1 μg/gm. Intra- and inter-assay variability was less than 8%. The development of the assay allowed the determination of fenbendazole in a live brine shrimp feeding study.

Original language	English (US)
Pages (from-to)	1536-1545
Number of pages	10
Journal	Journal of Liquid Chromatography and Related Technologies
Volume	34
Issue number	15
DOIs	https://doi.org/10.1080/10826076.2011.575977
State	Published - Sep 1 2011
Externally published	Yes

Keywords

HPLC
anthelmintics
aquaculture
artemia
bioencapsulation
fenbendazole

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Pharmaceutical Science
Clinical Biochemistry

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Cite this

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abstract = "A new HPLC procedure for the determination of fenbendazole, a benzimidazole, in adult Artemia (live brine shrimp) has been developed and validated. Following a liquid extraction using ethyl acetate, samples were separated by reversed-phase HPLC on a Sunfire C 18 column and quantified using UV detection at 215nm. The mobile phase was a mixture of 37.5mM potassium dihydrogen phosphate pH 3.75 and acetonitrile, with a flow-rate of 0.9mL/min. The procedure produced a linear curve over the concentration range of 100-5000μg/gm with a lower limit of quantification of 1 μg/gm. Intra- and inter-assay variability was less than 8%. The development of the assay allowed the determination of fenbendazole in a live brine shrimp feeding study.",

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