TY - JOUR
T1 - Detection of Trypanosoma brucei in field-captured tsetse flies and identification of host species fed on by the infected flies
AU - Konnai, Satoru
AU - Mekata, Hirohisa
AU - Odbileg, Raadan
AU - Simuunza, Martin
AU - Chembensof, Mwelwa
AU - Witola, William Harold
AU - Tembo, Mwase Enala
AU - Chitambo, Harrison
AU - Inoue, Noboru
AU - Onuma, Misao
AU - Ohashi, Kazuhiko
PY - 2008/8/1
Y1 - 2008/8/1
N2 - The prevalence of trypanosome infections in tsetse flies in the Chiawa area of Lower Zambezi in Zambia, with endemic trypanosomosis, was determined by a polymerase chain reaction (PCR) method that allowed the detection of trypanosome DNA and determination of the type of animal host fed on by the tsetse fly Glossina pallidipes, using tsetse-derived DNA extracts as templates. Ninety G. pallidipes (82 females and 8 males; 18.3%) of the 492 flies captured by baited biconical traps tested positive for the presence of Trypanosoma brucei species genomic DNA. Of the 90 T. brucei-positive flies, 47 (52.2%) also tested positive for vertebrate mitochondrial DNA. Sequence analysis of the vertebrate mitochondrial DNA amplicons established that they originated from 8 different vertebrate species, namely, human (Homo sapiens), African elephant (Loxodonta cyclotis), African buffalo (Syncerus caffer), waterbuck (Kobus ellipsiprymnus), roan antelope (Hippotragus equinus), greater kudu (Tragelaphus strepsiceros), warthog (Phacochoerus africanus), and goat (Capra hircus). Furthermore, to investigate the prevalence of trypanosome infections in domestic goats in the same area where trypanosomes had been detected in tsetse files, a total of 86 goats were randomly selected from 6 different herds. Among the selected goats, 36 (41.9%) were found to be positive for T. brucei species. This combined detection method would be an ideal approach not only for mass screening for infection prevalence in tsetse populations, but also for the prediction of natural reservoirs in areas endemic for trypanosomosis.
AB - The prevalence of trypanosome infections in tsetse flies in the Chiawa area of Lower Zambezi in Zambia, with endemic trypanosomosis, was determined by a polymerase chain reaction (PCR) method that allowed the detection of trypanosome DNA and determination of the type of animal host fed on by the tsetse fly Glossina pallidipes, using tsetse-derived DNA extracts as templates. Ninety G. pallidipes (82 females and 8 males; 18.3%) of the 492 flies captured by baited biconical traps tested positive for the presence of Trypanosoma brucei species genomic DNA. Of the 90 T. brucei-positive flies, 47 (52.2%) also tested positive for vertebrate mitochondrial DNA. Sequence analysis of the vertebrate mitochondrial DNA amplicons established that they originated from 8 different vertebrate species, namely, human (Homo sapiens), African elephant (Loxodonta cyclotis), African buffalo (Syncerus caffer), waterbuck (Kobus ellipsiprymnus), roan antelope (Hippotragus equinus), greater kudu (Tragelaphus strepsiceros), warthog (Phacochoerus africanus), and goat (Capra hircus). Furthermore, to investigate the prevalence of trypanosome infections in domestic goats in the same area where trypanosomes had been detected in tsetse files, a total of 86 goats were randomly selected from 6 different herds. Among the selected goats, 36 (41.9%) were found to be positive for T. brucei species. This combined detection method would be an ideal approach not only for mass screening for infection prevalence in tsetse populations, but also for the prediction of natural reservoirs in areas endemic for trypanosomosis.
KW - Glossina pallidipes
KW - Goat
KW - Host identification
KW - Trypanosoma brucei
KW - Zambia
UR - http://www.scopus.com/inward/record.url?scp=50849138809&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=50849138809&partnerID=8YFLogxK
U2 - 10.1089/vbz.2007.0223
DO - 10.1089/vbz.2007.0223
M3 - Article
C2 - 18399780
AN - SCOPUS:50849138809
SN - 1530-3667
VL - 8
SP - 565
EP - 573
JO - Vector-Borne and Zoonotic Diseases
JF - Vector-Borne and Zoonotic Diseases
IS - 4
ER -