Abstract
The single open reading frame (ORF) 5 encoding the 50-kDa protein of barley yellow dwarf virus PAV-IL (BYDV-PAV-IL) was expressed in bacteria, purified, and used as an immunogen/antigen to produce/screen antibodies specific to the 50-kDa protein. Two monoclonal antibodies (MAb PAV-IL-22 kDa and MAb PAV-IL-50 kDa) raised against BYDV-PAV-IL could specifically detect the presence of the 72-kDa readthrough protein in extracts from the BYDV- infected leaf tissue. The results suggest that ORF 5 (50-kDa protein) is translated by readthrough of ORF 3 (22-kDa coat protein) to produce the 72- kDa protein. The readthrough protein is thought to be a structural protein on the external surface of BYDV.
Original language | English (US) |
---|---|
Article number | 71427 |
Pages (from-to) | 1003-1006 |
Number of pages | 4 |
Journal | Virology |
Volume | 202 |
Issue number | 2 |
DOIs | |
State | Published - Aug 1 1994 |
Externally published | Yes |
ASJC Scopus subject areas
- Virology