Detection of silver ions using dielectrophoretic tweezers-based force spectroscopy

Seungyeop Choi, Gyudo Lee, In Soo Park, Myeonggu Son, Woong Kim, Hyungbeen Lee, Sei Young Lee, Sungsoo Na, Dae Sung Yoon, Rashid Bashir, Jinsung Park, Sang Woo Lee

Research output: Contribution to journalArticlepeer-review


Understanding of the interactions of silver ions (Ag+) with polynucleotides is important not only to detect Ag+ over a wide range of concentrations in a simple, robust, and high-throughput manner but also to investigate the intermolecular interactions of hydrogen and coordinate interactions that are generated due to the interplay of Ag+, hydrogen ions (H+), and polynucleotides since it is critical to prevent adverse environmental effects that may cause DNA damage and develop strategies to treat this damage. Here, we demonstrate a novel approach to simultaneously detect Ag+ satisfying the above requirements and examine the combined intermolecular interactions of Ag+-polycytosine and H+-polycytosine DNA complexes using dielectrophoretic tweezers-based force spectroscopy. For this investigation, we detected Ag+ over a range of concentrations (1 nM to 100 μM) by quantifying the rupture force of the combined interactions and examined the interplay between the three factors (Ag+, H+, and polycytosine) using the same assay for the detection of Ag+. Our study provides a new avenue not only for the detection of heavy metal ions but also for the investigation of heavy metal ions.polynucleotide DNA complexes using the same assay.

Original languageEnglish (US)
Pages (from-to)10867-10875
Number of pages9
JournalAnalytical Chemistry
Issue number22
StatePublished - Nov 15 2016

ASJC Scopus subject areas

  • Analytical Chemistry


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