Detection of Pyocyanin Using a New Biodegradable SERS Biosensor Fabricated Using Gold Coated Zein Nanostructures Further Decorated with Gold Nanoparticles

Fei Jia, Emma Barber, Hazal Turasan, Sujin Seo, Ruitong Dai, Gang Logan Liu, Xingmin Li, Arun K. Bhunia, Jozef L. Kokini

Research output: Contribution to journalArticle

Abstract

In this paper, a biodegradable gold coated zein film surface enhanced Raman spectroscopy (SERS) platform, with gold nanoparticles (AuNPs) deposited on the surface to further enhance the Raman signal, was used to detect pyocyanin (PYO), the toxin secreted by Pseudomonas aeruginosa. An inverted pyramid structure imprinted on a zein film and gold coated during the transfer process was further improved with the deposition and fixing of gold nanoparticles, which resulted in enhancement of the SERS signal by approximately a decade. This new platform served as a lab-on-a-chip sensor to enable the sensitive and rapid detection of PYO in drinking water. The size, distribution, and morphology of the zein film nanostructures including the presence and distribution of gold nanoparticles were characterized by scanning electron microscopy (SEM). The new zein-based platform has the advantage of being largely biodegradable compared with commercial silicon- or glass-based platforms. The limit of detection for PYO using the newly developed zein-based SERS sensor platform was calculated as 25 μM, considerably lower than the concentration of PYO in the blood of people with cystic fibrosis which has been reported to be 70 μM.

Original languageEnglish (US)
Pages (from-to)4603-4610
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume67
Issue number16
DOIs
StatePublished - Apr 24 2019

Fingerprint

Pyocyanine
Zein
nanomaterials
Raman spectroscopy
zein
biodegradability
Raman Spectrum Analysis
Nanostructures
biosensors
Biosensing Techniques
Biosensors
Gold
Nanoparticles
gold
films (materials)
sensors (equipment)
Lab-on-a-chip
cystic fibrosis
Sensors
Silicon

Keywords

  • SERS sensor
  • biodegradable
  • inverted pyramid structure
  • pyocyanin
  • zein film

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Detection of Pyocyanin Using a New Biodegradable SERS Biosensor Fabricated Using Gold Coated Zein Nanostructures Further Decorated with Gold Nanoparticles. / Jia, Fei; Barber, Emma; Turasan, Hazal; Seo, Sujin; Dai, Ruitong; Liu, Gang Logan; Li, Xingmin; Bhunia, Arun K.; Kokini, Jozef L.

In: Journal of Agricultural and Food Chemistry, Vol. 67, No. 16, 24.04.2019, p. 4603-4610.

Research output: Contribution to journalArticle

Jia, Fei ; Barber, Emma ; Turasan, Hazal ; Seo, Sujin ; Dai, Ruitong ; Liu, Gang Logan ; Li, Xingmin ; Bhunia, Arun K. ; Kokini, Jozef L. / Detection of Pyocyanin Using a New Biodegradable SERS Biosensor Fabricated Using Gold Coated Zein Nanostructures Further Decorated with Gold Nanoparticles. In: Journal of Agricultural and Food Chemistry. 2019 ; Vol. 67, No. 16. pp. 4603-4610.
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