Detection of lipopolysaccharide components in isolated outer membranes of Serratia marcescens

J. C. Tsang, D. A. Brown, David M Kranz

Research output: Contribution to journalArticle

Abstract

The lipopolysaccharide (LPS) component in outer membranes isolated from two strains of S. marcescens was specifically detected and identified by a staining technique using carbocyanine dye after polyacrylamide electrophoresis. Two LPS containing components were found in the outer membrane fractions. The major component was shown to have an apparent molecular weight of 12,000 daltons and was associated with a protein component of the outer membrane. The carbocyanine dye was found to be more sensitive and specific for LPS than other common stains used for the detection of carbohydrates and proteins. The potential of this technique for the quantitative measurement of the LPS content directly from outer membranes was discussed.

Original languageEnglish (US)
Pages (from-to)209-217
Number of pages9
JournalMicrobios Letters
Volume1
Issue number3-4
StatePublished - Jan 1 1976
Externally publishedYes

Fingerprint

Serratia marcescens
Lipopolysaccharides
Carbocyanines
Membranes
Coloring Agents
Electrophoresis
Proteins
Molecular Weight
Molecular weight
Carbohydrates
Staining and Labeling

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Hematology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology

Cite this

Detection of lipopolysaccharide components in isolated outer membranes of Serratia marcescens. / Tsang, J. C.; Brown, D. A.; Kranz, David M.

In: Microbios Letters, Vol. 1, No. 3-4, 01.01.1976, p. 209-217.

Research output: Contribution to journalArticle

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