Detection of latent pseudorabies virus in swine using in situ hybridization

T. M. Brown, F. A. Osorio, D. L. Rock

Research output: Contribution to journalArticlepeer-review


We have examined methods for detection of pseudorabies virus (PRV) latency in three groups of swine; naturally infected animals obtained from a field case; animals which have been experimentally infected with Becker or Iowa strains of PRV; and single reactors (single seropositive animals within PRV-free herds). In situ hybridization was shown to be more sensitive than explanation/co-cultivation for the detection of latent virus. Nervous tissues, in particular the trigeminal ganglia, were found to be the most reliable source for detecting latent PRV. The presence of latent PRV was not detected in lymphoid tissues examined.

Original languageEnglish (US)
Pages (from-to)273-280
Number of pages8
JournalVeterinary Microbiology
Issue number3-4
StatePublished - Sep 1990
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)


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