Detection of inverted beet sugar adulteration of honey by FTIR spectroscopy

S. Sivakesava, J. Irudayaraj

Research output: Contribution to journalArticlepeer-review


A combination of Fourier transform infrared (FTIR) spectroscopy and multivariate statistics as a screening tool for the determination of beet medium invert sugar adulteration in three different varieties of honey is discussed. Honey samples with different concentrations of beet invert sugar were scanned using the attenuated total reflectance (ATR) accessory of the Bio-Rad FTS-6000 Fourier transform spectrometer. The spectral wavenumber region between 950 and 1500cm-1 was selected for partial least squares (PLS) regression to develop calibration models for beet invert sugar determination in honey samples. Results from the PLS (first derivative) models were slightly better than those obtained with other calibration models. Predictive models were also developed to classify beet sugar invert in three different varieties of honey samples using discriminant analysis. Spectral data were compressed using the principal component method, and linear discriminant and canonical variate analyses were used to detect the level of beet invert sugar in honey samples. The best predictive model for adulterated honey samples was achieved with canonical variate analysis, which successfully classified 88-94 per cent of the validation set. The present study demonstrated that Fourier transform infrared spectroscopy could be used for rapid detection of beet invert sugar adulteration in different varieties of honey.

Original languageEnglish (US)
Pages (from-to)683-690
Number of pages8
JournalJournal of the Science of Food and Agriculture
Issue number8
StatePublished - Apr 10 2001
Externally publishedYes


  • Adulteration
  • Beet invert sugar
  • Chemometrics
  • Discriminant analysis
  • Fourier transform infrared spectroscopy
  • Honey

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Agronomy and Crop Science
  • Nutrition and Dietetics


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