Detection of intraspecific DNA content variation in Zea mays L. by flow cytometry

A Lane Rayburn, Julie A. Auger, Elizabeth A. Benzinger, Angus G. Hepburn

Research output: Contribution to journalArticle

Abstract

Flow cytometry was used to determine quantitative intraspecific DNA content variation in Zea mays. Previous studies using flow cytometry had indicated that intraspecific variation in corn was beyond the resolution of the method. The DNA content variation among corn lines observed in this study was in agreement with the amount of variation observed using microdensitometry. In order to observe intraspecific variation, the fluorochrome DAPI was shown to be superior to mithramycin. The fluorochrome: nuclei ratio was found to be critical when DAPI was used because of self absorption of the fluorescence. Flow cytometry with the fluorochrome DAPI was found to be a rapid and reliable alternative to microdensitometry in examining intraspecific DNA content variation in corn.

Original languageEnglish (US)
Pages (from-to)1179-1183
Number of pages5
JournalJournal of experimental botany
Volume40
Issue number11
DOIs
StatePublished - Nov 1 1989

Fingerprint

Zea Mays L.
Flow Cytometry
Flow cytometry
fluorescent dyes
flow cytometry
Zea mays
DNA
maize
Fluorescent Dyes
intraspecific variation
Corn
corn
Plicamycin
fluorescence
Fluorescence
Zea Mays
Nucleus
detection
Absorption
DAPI

Keywords

  • Corn (maize)
  • DAPI
  • Genome size
  • Zea mays

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Cite this

Detection of intraspecific DNA content variation in Zea mays L. by flow cytometry. / Rayburn, A Lane; Auger, Julie A.; Benzinger, Elizabeth A.; Hepburn, Angus G.

In: Journal of experimental botany, Vol. 40, No. 11, 01.11.1989, p. 1179-1183.

Research output: Contribution to journalArticle

Rayburn, A Lane ; Auger, Julie A. ; Benzinger, Elizabeth A. ; Hepburn, Angus G. / Detection of intraspecific DNA content variation in Zea mays L. by flow cytometry. In: Journal of experimental botany. 1989 ; Vol. 40, No. 11. pp. 1179-1183.
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