Detection of HSV-1 genome in central nervous system of latently infected mice

Daniel L. Rock, Nigel W. Fraser

Research output: Contribution to journalArticlepeer-review

Abstract

Herpes simplex virus type 1 (HSV-1) can establish latent infections in peripheral nerve ganglia and the central nervous system (CNS) of experimentally infected mice. Latent infections of peripheral nervous tissue are characterized by the ability to recover infectious virus from explant cultures of most of the latently infected ganglia1-4. In contrast, infectious virus is infrequently recovered from the CNS of latently infected mice following explant culture2-4, although viral DNA can be detected in CNS tissue 4. The presence of incomplete or defective viral genomes during the latent CNS infections has been proposed to explain this difference between the two latent infections4. To characterize the completeness and the physical state of the latent viral genome, we used Southern blot hybridization to analyse the viral DNA from latently infected mouse ganglia and brains. Our results, reported here, indicate that most, if not all, of the HSV-1 genome is present in latently infected CNS tissue and that the latent viral genome exists in a form other than linear, unit length DNA.

Original languageEnglish (US)
Pages (from-to)523-525
Number of pages3
JournalNature
Volume302
Issue number5908
DOIs
StatePublished - 1983
Externally publishedYes

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Detection of HSV-1 genome in central nervous system of latently infected mice'. Together they form a unique fingerprint.

Cite this