Detection of glucose, galactose, and lactose in milk with a microdialysis-coupled flow injection amperometric sensor

V. Rajendran, J. Irudayaraj

Research output: Contribution to journalArticlepeer-review

Abstract

A microdialysis-coupled flow injection amperometric Sensor (μFIAS) was used to determine glucose, galactose, and lactose in milk. The sensor is based on enzyme-catalyzed reaction in combination with the three well-established analytical techniques, namely; microdialysis sampling, flow injection analysis (FIA), and amperometric detection. With the multianalyte sensor it was possible to detect glucose and galactose by sequential injection of their corresponding oxidase enzymes: glucose oxidase and galactose oxidase, while lactose was determined by injection of a mixture of beta-galactosidase and glucose oxidase enzymes. The sensor showed a linear response between 0.05 and 10 mM for glucose, between 0.1 and 20 mM for galactose and between 0.2 and 20 mM for lactose, respectively. The relative standard deviation values of the sensor measurements for glucose, galactose, and lactose were 3-4% (n = 3). The sensor measurements for lactose content in milk were compared with a standard method with an infrared spectrophotometer.

Original languageEnglish (US)
Pages (from-to)1357-1361
Number of pages5
JournalJournal of Dairy Science
Volume85
Issue number6
DOIs
StatePublished - Jun 2002
Externally publishedYes

Keywords

  • Flow injection
  • Lactose
  • Milk
  • Sensor

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics

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