TY - JOUR
T1 - Detection of glucose, galactose, and lactose in milk with a microdialysis-coupled flow injection amperometric sensor
AU - Rajendran, V.
AU - Irudayaraj, J.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2002/6
Y1 - 2002/6
N2 - A microdialysis-coupled flow injection amperometric Sensor (μFIAS) was used to determine glucose, galactose, and lactose in milk. The sensor is based on enzyme-catalyzed reaction in combination with the three well-established analytical techniques, namely; microdialysis sampling, flow injection analysis (FIA), and amperometric detection. With the multianalyte sensor it was possible to detect glucose and galactose by sequential injection of their corresponding oxidase enzymes: glucose oxidase and galactose oxidase, while lactose was determined by injection of a mixture of beta-galactosidase and glucose oxidase enzymes. The sensor showed a linear response between 0.05 and 10 mM for glucose, between 0.1 and 20 mM for galactose and between 0.2 and 20 mM for lactose, respectively. The relative standard deviation values of the sensor measurements for glucose, galactose, and lactose were 3-4% (n = 3). The sensor measurements for lactose content in milk were compared with a standard method with an infrared spectrophotometer.
AB - A microdialysis-coupled flow injection amperometric Sensor (μFIAS) was used to determine glucose, galactose, and lactose in milk. The sensor is based on enzyme-catalyzed reaction in combination with the three well-established analytical techniques, namely; microdialysis sampling, flow injection analysis (FIA), and amperometric detection. With the multianalyte sensor it was possible to detect glucose and galactose by sequential injection of their corresponding oxidase enzymes: glucose oxidase and galactose oxidase, while lactose was determined by injection of a mixture of beta-galactosidase and glucose oxidase enzymes. The sensor showed a linear response between 0.05 and 10 mM for glucose, between 0.1 and 20 mM for galactose and between 0.2 and 20 mM for lactose, respectively. The relative standard deviation values of the sensor measurements for glucose, galactose, and lactose were 3-4% (n = 3). The sensor measurements for lactose content in milk were compared with a standard method with an infrared spectrophotometer.
KW - Flow injection
KW - Lactose
KW - Milk
KW - Sensor
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U2 - 10.3168/jds.S0022-0302(02)74201-X
DO - 10.3168/jds.S0022-0302(02)74201-X
M3 - Article
C2 - 12146464
AN - SCOPUS:0036615580
SN - 0022-0302
VL - 85
SP - 1357
EP - 1361
JO - Journal of Dairy Science
JF - Journal of Dairy Science
IS - 6
ER -