Detection of Ehrlichia canis by PCR in different tissues obtained during necropsy from dogs surveyed for naturally occurring canine monocytic ehrlichiosis

Arnon Gal, Emmanuel Loeb, Yael Yisaschar-Mekuzas, Gad Baneth

Research output: Contribution to journalArticle

Abstract

A molecular study for the detection of Ehrlichia canis was carried out on tissues obtained at necropsy from randomly selected dogs with the intention of investigating naturally-occurring canine ehrlichiosis. The tissues evaluated for the presence of E. canis included lymph nodes, spleen, liver, bone marrow, and blood. Eight of the 18 dogs included were found to be positive for E. canis by polymerase chain reaction (PCR) and sequencing of the 16S rRNA gene. Two dogs were positive for Anaplasma platys of which one dog was co-infected with E. canis and A. platys. Blood (5/8) and lymph nodes (5/8) were the tissues found to yield the highest number of positive E. canis PCR results with 7/8 dogs positive in the blood or lymph node. E. canis and A. platys DNA could be amplified by PCR when tissue samples were obtained 72 h after the time of death.

Original languageEnglish (US)
Pages (from-to)212-217
Number of pages6
JournalVeterinary Journal
Volume175
Issue number2
DOIs
StatePublished - Feb 1 2008
Externally publishedYes

Keywords

  • Anaplasma platys
  • Ehrlichia canis
  • Necropsy
  • PCR
  • Tissue

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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