TY - JOUR
T1 - Detection and Quantification of Myocardial Fibrosis Using Stain-Free Infrared Spectroscopic Imaging
AU - Zimmermann, Eric
AU - Mukherjee, Sudipta S
AU - Falahkheirkhah, Kianoush
AU - Gryka, Mark C
AU - Kajdacsy-Balla, Andre
AU - Hasan, Wohaib
AU - Giraud, George
AU - Tibayan, Fred
AU - Raman, Jai
AU - Bhargava, Rohit
N1 - Funding Information:
This work uses resources provided by the Innovative Systems Laboratory at the National Center for Supercomputing Applications at the University of Illinois at Urbana-Champaign. Dr Zimmermann would like to thank the National Institutes of Health, T32 training grant NIH NRSA T32HL094294 for support.
Publisher Copyright:
© 2021 College of American Pathologists. All rights reserved.
PY - 2021/12
Y1 - 2021/12
N2 - Context.-Myocardial fibrosis underpins a number of cardiovascular conditions and is difficult to identify with standard histologic techniques. Challenges include imaging, defining an objective threshold for classifying fibrosis as mild or severe, and understanding the molecular basis for these changes. Objective.-To develop a novel, rapid, label-free approach to accurately measure and quantify the extent of fibrosis in cardiac tissue using infrared spectroscopic imaging. Design.-We performed infrared spectroscopic imaging and combined that with advanced machine learning-based algorithms to assess fibrosis in 15 samples from patients belonging to the following 3 classes: (1) patients with nonpathologic (control) donor hearts, (2) patients undergoing transplant, and (3) patients undergoing implantation of a ventricular assist device. Results.-Our results show excellent sensitivity and accuracy for detecting myocardial fibrosis, as demonstrated by a high area under the curve of 0.998 in the receiver operating characteristic curve measured from infrared imaging. Fibrosis of various morphologic subtypes were demonstrated with virtually generated picrosirius red images, which showed good visual and quantitative agreement (correlation coefficient = 0.92, q = 7.76 3 10_15) with stained images of the same sections. Underlying molecular composition of the different subtypes was investigated with infrared spectra showing reproducible differences presumably arising from differences in collagen subtypes and/or crosslinking. Conclusions.-Infrared imaging can be a powerful tool in studying myocardial fibrosis and gleaning insights into the underlying chemical changes that accompany it. Emerging methods suggest that the proposed approach is compatible with conventional optical microscopy, and its consistency makes it translatable to the clinical setting for real-time diagnoses as well as for objective and quantitative research.
AB - Context.-Myocardial fibrosis underpins a number of cardiovascular conditions and is difficult to identify with standard histologic techniques. Challenges include imaging, defining an objective threshold for classifying fibrosis as mild or severe, and understanding the molecular basis for these changes. Objective.-To develop a novel, rapid, label-free approach to accurately measure and quantify the extent of fibrosis in cardiac tissue using infrared spectroscopic imaging. Design.-We performed infrared spectroscopic imaging and combined that with advanced machine learning-based algorithms to assess fibrosis in 15 samples from patients belonging to the following 3 classes: (1) patients with nonpathologic (control) donor hearts, (2) patients undergoing transplant, and (3) patients undergoing implantation of a ventricular assist device. Results.-Our results show excellent sensitivity and accuracy for detecting myocardial fibrosis, as demonstrated by a high area under the curve of 0.998 in the receiver operating characteristic curve measured from infrared imaging. Fibrosis of various morphologic subtypes were demonstrated with virtually generated picrosirius red images, which showed good visual and quantitative agreement (correlation coefficient = 0.92, q = 7.76 3 10_15) with stained images of the same sections. Underlying molecular composition of the different subtypes was investigated with infrared spectra showing reproducible differences presumably arising from differences in collagen subtypes and/or crosslinking. Conclusions.-Infrared imaging can be a powerful tool in studying myocardial fibrosis and gleaning insights into the underlying chemical changes that accompany it. Emerging methods suggest that the proposed approach is compatible with conventional optical microscopy, and its consistency makes it translatable to the clinical setting for real-time diagnoses as well as for objective and quantitative research.
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U2 - 10.5858/arpa.2020-0635-OA
DO - 10.5858/arpa.2020-0635-OA
M3 - Article
C2 - 33755723
SN - 0003-9985
VL - 145
SP - 1526
EP - 1535
JO - Archives of Pathology and Laboratory Medicine
JF - Archives of Pathology and Laboratory Medicine
IS - 12
ER -