Detecting and sorting methylated DNA using a synthetic nanopore

Winston Timp; Andrew Feinberg; Utkur M. Mirsaidov; Gregory Timp

Detecting and sorting methylated DNA using a synthetic nanopore

Winston Timp (Inventor)
, Andrew Feinberg (Inventor)
, Utkur M. Mirsaidov (Inventor)
, Gregory Timp (Inventor)

University of Illinois Urbana-Champaign

Research output: Patent

Abstract

Provided are methods for detecting, characterizing or separating DNA based on methylation. Heterogeneous DNA populations are separated based on DNA methylation by providing a membrane having a nanopore through which an electric field is applied. DNA of interest is introduced, and for a given threshold voltage across the nanopore, only DNA having a methylation parameter of interest may transit the pore, thereby facilitating detection, characterization, or separation of DNA based on methylation. The methods are optionally used to detect a disease state that is associated with DNA methylation including, but not limited to, cancer.

Original language	English (US)
U.S. patent number	8394584
Filing date	12/18/09
State	Published - Mar 12 2013

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title = "Detecting and sorting methylated DNA using a synthetic nanopore",

abstract = "Provided are methods for detecting, characterizing or separating DNA based on methylation. Heterogeneous DNA populations are separated based on DNA methylation by providing a membrane having a nanopore through which an electric field is applied. DNA of interest is introduced, and for a given threshold voltage across the nanopore, only DNA having a methylation parameter of interest may transit the pore, thereby facilitating detection, characterization, or separation of DNA based on methylation. The methods are optionally used to detect a disease state that is associated with DNA methylation including, but not limited to, cancer.",

author = "Winston Timp and Andrew Feinberg and Mirsaidov, \{Utkur M.\} and Gregory Timp",

note = "STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT This invention was made with government support under RO1 HG003713A awarded by the National Institute of Health. The government has certain rights in the invention.; 8394584",

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day = "12",

language = "English (US)",

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AU - Timp, Winston

AU - Feinberg, Andrew

AU - Mirsaidov, Utkur M.

AU - Timp, Gregory

N1 - STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT This invention was made with government support under RO1 HG003713A awarded by the National Institute of Health. The government has certain rights in the invention.

PY - 2013/3/12

Y1 - 2013/3/12

N2 - Provided are methods for detecting, characterizing or separating DNA based on methylation. Heterogeneous DNA populations are separated based on DNA methylation by providing a membrane having a nanopore through which an electric field is applied. DNA of interest is introduced, and for a given threshold voltage across the nanopore, only DNA having a methylation parameter of interest may transit the pore, thereby facilitating detection, characterization, or separation of DNA based on methylation. The methods are optionally used to detect a disease state that is associated with DNA methylation including, but not limited to, cancer.

AB - Provided are methods for detecting, characterizing or separating DNA based on methylation. Heterogeneous DNA populations are separated based on DNA methylation by providing a membrane having a nanopore through which an electric field is applied. DNA of interest is introduced, and for a given threshold voltage across the nanopore, only DNA having a methylation parameter of interest may transit the pore, thereby facilitating detection, characterization, or separation of DNA based on methylation. The methods are optionally used to detect a disease state that is associated with DNA methylation including, but not limited to, cancer.

M3 - Patent

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Y2 - 2009/12/18

ER -

Detecting and sorting methylated DNA using a synthetic nanopore

Abstract

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