Detecting and sorting methylated DNA using a synthetic nanopore

Gregory Timp; Winston Timp; Andrew Feinberg; Utkur M Mirsaidov

Detecting and sorting methylated DNA using a synthetic nanopore

Gregory Timp (Inventor), Winston Timp (Inventor), Andrew Feinberg (Inventor), Utkur M Mirsaidov (Inventor)

Research output: Patent

Abstract

Provided are methods for detecting, characterizing or separating DNA based on methylation. Heterogeneous DNA populations are separated based on DNA methylation by providing a membrane having a nanopore through which an electric field is applied. DNA of interest is introduced, and for a given threshold voltage across the nanopore, only DNA having a methylation parameter of interest may transit the pore, thereby facilitating detection, characterization, or separation of DNA based on methylation. The methods are optionally used to detect a disease state that is associated with DNA methylation including, but not limited to, cancer.

Original language	English (US)
U.S. patent number	8394584
Filing date	12/18/09
State	Published - Mar 12 2013

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title = "Detecting and sorting methylated DNA using a synthetic nanopore",

abstract = "Provided are methods for detecting, characterizing or separating DNA based on methylation. Heterogeneous DNA populations are separated based on DNA methylation by providing a membrane having a nanopore through which an electric field is applied. DNA of interest is introduced, and for a given threshold voltage across the nanopore, only DNA having a methylation parameter of interest may transit the pore, thereby facilitating detection, characterization, or separation of DNA based on methylation. The methods are optionally used to detect a disease state that is associated with DNA methylation including, but not limited to, cancer.",

author = "Gregory Timp and Winston Timp and Andrew Feinberg and Mirsaidov, {Utkur M}",

note = "STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT This invention was made with government support under RO1 HG003713A awarded by the National Institute of Health. The government has certain rights in the invention.; 8394584",

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AU - Timp, Gregory

AU - Timp, Winston

AU - Feinberg, Andrew

AU - Mirsaidov, Utkur M

N1 - STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT This invention was made with government support under RO1 HG003713A awarded by the National Institute of Health. The government has certain rights in the invention.

PY - 2013/3/12

Y1 - 2013/3/12

N2 - Provided are methods for detecting, characterizing or separating DNA based on methylation. Heterogeneous DNA populations are separated based on DNA methylation by providing a membrane having a nanopore through which an electric field is applied. DNA of interest is introduced, and for a given threshold voltage across the nanopore, only DNA having a methylation parameter of interest may transit the pore, thereby facilitating detection, characterization, or separation of DNA based on methylation. The methods are optionally used to detect a disease state that is associated with DNA methylation including, but not limited to, cancer.

AB - Provided are methods for detecting, characterizing or separating DNA based on methylation. Heterogeneous DNA populations are separated based on DNA methylation by providing a membrane having a nanopore through which an electric field is applied. DNA of interest is introduced, and for a given threshold voltage across the nanopore, only DNA having a methylation parameter of interest may transit the pore, thereby facilitating detection, characterization, or separation of DNA based on methylation. The methods are optionally used to detect a disease state that is associated with DNA methylation including, but not limited to, cancer.

M3 - Patent

M1 - 8394584

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ER -

Detecting and sorting methylated DNA using a synthetic nanopore

Abstract

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