Design of a microfabricated cell straining device for investigating cellular response to finite mechanical strains

J. C. Selby, M. A. Shannon, S. A. Boppart

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A methodology is presented for the design of a microfabricated blister-type device to examine the cellular response of isolated cells and multi-cellular aggregates to finite mechanical strains (1-5%):. The proposed device utilizes microfabrication technologies that incorporate the bonding, transfer, and assembly of thin (3 μm < thickness < 100 μm) elastic membranes onto rigid substrates with a circular hole or array of circular holes in the formation of diaphragm structures. An analysis of membrane deformations and strain fields of macro- and micro-scale polydimethylsiloxane (PDMS) diaphragm, blister-type devices demonstrates that microfabricated diaphragms can achieve finite strains corresponding to smaller axial displacements than those required for identical strains in macro-scale devices. Smaller axial displacements may decrease the confounding shear loading of the cell culture by the overlying nutrient medium. Additionally, it is shown that the uniform residual stress of a microfabricated diaphragm can be experimentally determined and utilized for more precise control of the incremental finite strains imposed on the diaphragm. Thus, microfabricated blister-type cell straining devices may potentially enhance future quantitative investigations of dynamic mechanically stimulated cell culture.

Original languageEnglish (US)
Title of host publication2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings
EditorsDavid Beebe, Andre Dittmar
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages174-179
Number of pages6
ISBN (Electronic)0780374800, 9780780374805
DOIs
StatePublished - Jan 1 2002
Event2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Madison, United States
Duration: May 2 2002May 4 2002

Publication series

Name2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings

Other

Other2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology
CountryUnited States
CityMadison
Period5/2/025/4/02

Fingerprint

Diaphragm
Equipment and Supplies
Blister
Cell Culture Techniques
Microtechnology
Membranes
Technology
Food

Keywords

  • cellular mechanotransduction
  • mechanostimulus

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology (medical)

Cite this

Selby, J. C., Shannon, M. A., & Boppart, S. A. (2002). Design of a microfabricated cell straining device for investigating cellular response to finite mechanical strains. In D. Beebe, & A. Dittmar (Eds.), 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings (pp. 174-179). [1002308] (2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MMB.2002.1002308

Design of a microfabricated cell straining device for investigating cellular response to finite mechanical strains. / Selby, J. C.; Shannon, M. A.; Boppart, S. A.

2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings. ed. / David Beebe; Andre Dittmar. Institute of Electrical and Electronics Engineers Inc., 2002. p. 174-179 1002308 (2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Selby, JC, Shannon, MA & Boppart, SA 2002, Design of a microfabricated cell straining device for investigating cellular response to finite mechanical strains. in D Beebe & A Dittmar (eds), 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings., 1002308, 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings, Institute of Electrical and Electronics Engineers Inc., pp. 174-179, 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology, Madison, United States, 5/2/02. https://doi.org/10.1109/MMB.2002.1002308
Selby JC, Shannon MA, Boppart SA. Design of a microfabricated cell straining device for investigating cellular response to finite mechanical strains. In Beebe D, Dittmar A, editors, 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings. Institute of Electrical and Electronics Engineers Inc. 2002. p. 174-179. 1002308. (2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings). https://doi.org/10.1109/MMB.2002.1002308
Selby, J. C. ; Shannon, M. A. ; Boppart, S. A. / Design of a microfabricated cell straining device for investigating cellular response to finite mechanical strains. 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings. editor / David Beebe ; Andre Dittmar. Institute of Electrical and Electronics Engineers Inc., 2002. pp. 174-179 (2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings).
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