Deregulation of glutamine PRPP amidotransferase activity

Janet L. Smith (Inventor), Robert L. Switzer (Inventor), Howard Zalkin (Inventor)

Research output: Patent


The present invention is directed to a modified amidotransferase. The amino acid sequence of the modified amidotransferase differs from the amino acid sequence of a native E. coli glutamine PRPP amidotransferase in that one or more amino acid residues of the modified amidotransferase are substituted at positions equivalent to amino acid positions in Bacillus amidotransferase selected from the group consisting of 282, 283, 307, and 347 of SEQ ID NO:1, wherein the modified amidotransferase is less sensitive to end-product inhibition than is the native E. coli glutamine PRPP amidotransferase.
Original languageEnglish (US)
U.S. patent number6586216
Filing date3/13/01
StatePublished - Jul 1 2003


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