Deregulation of glutamine PRPP amidotransferase activity

Janet L. Smith; Robert L. Switzer; Howard Zalkin

Deregulation of glutamine PRPP amidotransferase activity

Janet L. Smith (Inventor), Robert L. Switzer (Inventor), Howard Zalkin (Inventor)

Biochemistry

Research output: Patent

Abstract

The present invention is directed to a modified amidotransferase. The amino acid sequence of the modified amidotransferase differs from the amino acid sequence of a native E. coli glutamine PRPP amidotransferase in that one or more amino acid residues of the modified amidotransferase are substituted at positions equivalent to amino acid positions in Bacillus amidotransferase selected from the group consisting of 282, 283, 307, and 347 of SEQ ID NO:1, wherein the modified amidotransferase is less sensitive to end-product inhibition than is the native E. coli glutamine PRPP amidotransferase.

Original language	English (US)
U.S. patent number	6586216
Filing date	3/13/01
State	Published - Jul 1 2003

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title = "Deregulation of glutamine PRPP amidotransferase activity",

abstract = "The present invention is directed to a modified amidotransferase. The amino acid sequence of the modified amidotransferase differs from the amino acid sequence of a native E. coli glutamine PRPP amidotransferase in that one or more amino acid residues of the modified amidotransferase are substituted at positions equivalent to amino acid positions in Bacillus amidotransferase selected from the group consisting of 282, 283, 307, and 347 of SEQ ID NO:1, wherein the modified amidotransferase is less sensitive to end-product inhibition than is the native E. coli glutamine PRPP amidotransferase.",

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TY - PAT

T1 - Deregulation of glutamine PRPP amidotransferase activity

AU - Smith, Janet L.

AU - Switzer, Robert L.

AU - Zalkin, Howard

PY - 2003/7/1

Y1 - 2003/7/1

N2 - The present invention is directed to a modified amidotransferase. The amino acid sequence of the modified amidotransferase differs from the amino acid sequence of a native E. coli glutamine PRPP amidotransferase in that one or more amino acid residues of the modified amidotransferase are substituted at positions equivalent to amino acid positions in Bacillus amidotransferase selected from the group consisting of 282, 283, 307, and 347 of SEQ ID NO:1, wherein the modified amidotransferase is less sensitive to end-product inhibition than is the native E. coli glutamine PRPP amidotransferase.

AB - The present invention is directed to a modified amidotransferase. The amino acid sequence of the modified amidotransferase differs from the amino acid sequence of a native E. coli glutamine PRPP amidotransferase in that one or more amino acid residues of the modified amidotransferase are substituted at positions equivalent to amino acid positions in Bacillus amidotransferase selected from the group consisting of 282, 283, 307, and 347 of SEQ ID NO:1, wherein the modified amidotransferase is less sensitive to end-product inhibition than is the native E. coli glutamine PRPP amidotransferase.

M3 - Patent

M1 - 6586216

Y2 - 2001/03/13

ER -

Deregulation of glutamine PRPP amidotransferase activity

Abstract

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