The present invention is directed to a modified glutamine PRPP amidotransferase and a method of using that modified enzyme to enhance the biosynthesis of purine nucleotides. The modified glutamine PRPP amidotransferase has at least one amino acid of the allosteric A sites or the catalytic C sites of said amidotransferase substituted with a non-native amino acid, wherein the substitution reduces the sensitivity of the enzyme to end product inhibition relative to the native glutamine PRPP amidotransferase enzyme.
|U.S. patent number
|Published - Mar 20 2001