Deregulation of glutamine PRPP amidotransferase activity

Janet L. Smith; Robert L. Switzer; Howard Zalkin

Deregulation of glutamine PRPP amidotransferase activity

Janet L. Smith (Inventor), Robert L. Switzer (Inventor), Howard Zalkin (Inventor)

Biochemistry

Research output: Patent

Abstract

The present invention is directed to a modified glutamine PRPP amidotransferase and a method of using that modified enzyme to enhance the biosynthesis of purine nucleotides. The modified glutamine PRPP amidotransferase has at least one amino acid of the allosteric A sites or the catalytic C sites of said amidotransferase substituted with a non-native amino acid, wherein the substitution reduces the sensitivity of the enzyme to end product inhibition relative to the native glutamine PRPP amidotransferase enzyme.

Original language	English (US)
U.S. patent number	6204041
Filing date	9/9/98
State	Published - Mar 20 2001

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title = "Deregulation of glutamine PRPP amidotransferase activity",

abstract = "The present invention is directed to a modified glutamine PRPP amidotransferase and a method of using that modified enzyme to enhance the biosynthesis of purine nucleotides. The modified glutamine PRPP amidotransferase has at least one amino acid of the allosteric A sites or the catalytic C sites of said amidotransferase substituted with a non-native amino acid, wherein the substitution reduces the sensitivity of the enzyme to end product inhibition relative to the native glutamine PRPP amidotransferase enzyme.",

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year = "2001",

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day = "20",

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TY - PAT

T1 - Deregulation of glutamine PRPP amidotransferase activity

AU - Smith, Janet L.

AU - Switzer, Robert L.

AU - Zalkin, Howard

PY - 2001/3/20

Y1 - 2001/3/20

N2 - The present invention is directed to a modified glutamine PRPP amidotransferase and a method of using that modified enzyme to enhance the biosynthesis of purine nucleotides. The modified glutamine PRPP amidotransferase has at least one amino acid of the allosteric A sites or the catalytic C sites of said amidotransferase substituted with a non-native amino acid, wherein the substitution reduces the sensitivity of the enzyme to end product inhibition relative to the native glutamine PRPP amidotransferase enzyme.

AB - The present invention is directed to a modified glutamine PRPP amidotransferase and a method of using that modified enzyme to enhance the biosynthesis of purine nucleotides. The modified glutamine PRPP amidotransferase has at least one amino acid of the allosteric A sites or the catalytic C sites of said amidotransferase substituted with a non-native amino acid, wherein the substitution reduces the sensitivity of the enzyme to end product inhibition relative to the native glutamine PRPP amidotransferase enzyme.

M3 - Patent

M1 - 6204041

Y2 - 1998/09/09

ER -

Deregulation of glutamine PRPP amidotransferase activity

Abstract

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