Depth-resolved image mapping spectrometer (IMS) with structured illumination

Liang Gao, Noah Bedard, Nathan Hagen, Robert T. Kester, Tomasz S. Tkaczyk

Research output: Contribution to journalArticlepeer-review

Abstract

We present a depth-resolved Image Mapping Spectrometer (IMS) which is capable of acquiring 4D (x, y, z, λ) datacubes. Optical sectioning is implemented by structured illumination. The device's spectral imaging performance is demonstrated in a multispectral microsphere and mouse kidney tissue fluorescence imaging experiment. We also compare quantitatively the depth-resolved IMS with a hyperspectral confocal microscope (HCM) in a standard fluorescent bead imaging experiment. The comparison results show that despite the use of a light source with four orders of magnitude lower intensity in the IMS than that in the HCM, the image signal-to-noise ratio acquired by the IMS is 2.6 times higher than that achieved by the equivalent confocal approach.

Original languageEnglish (US)
Pages (from-to)17439-17452
Number of pages14
JournalOptics Express
Volume19
Issue number18
DOIs
StatePublished - Aug 29 2011

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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