Depth-resolved image mapping spectrometer (IMS) with structured illumination

Liang Gao; Noah Bedard; Nathan Hagen; Robert T. Kester; Tomasz S. Tkaczyk

doi:10.1364/OE.19.017439

Depth-resolved image mapping spectrometer (IMS) with structured illumination

Liang Gao, Noah Bedard, Nathan Hagen, Robert T. Kester, Tomasz S. Tkaczyk

Research output: Contribution to journal › Article › peer-review

Abstract

We present a depth-resolved Image Mapping Spectrometer (IMS) which is capable of acquiring 4D (x, y, z, λ) datacubes. Optical sectioning is implemented by structured illumination. The device's spectral imaging performance is demonstrated in a multispectral microsphere and mouse kidney tissue fluorescence imaging experiment. We also compare quantitatively the depth-resolved IMS with a hyperspectral confocal microscope (HCM) in a standard fluorescent bead imaging experiment. The comparison results show that despite the use of a light source with four orders of magnitude lower intensity in the IMS than that in the HCM, the image signal-to-noise ratio acquired by the IMS is 2.6 times higher than that achieved by the equivalent confocal approach.

Original language	English (US)
Pages (from-to)	17439-17452
Number of pages	14
Journal	Optics Express
Volume	19
Issue number	18
DOIs	https://doi.org/10.1364/OE.19.017439
State	Published - Aug 29 2011
Externally published	Yes

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics

Access to Document

10.1364/OE.19.017439

Cite this

@article{942aaf31ecf243bfa442e4f5a7c0b31b,

title = "Depth-resolved image mapping spectrometer (IMS) with structured illumination",

abstract = "We present a depth-resolved Image Mapping Spectrometer (IMS) which is capable of acquiring 4D (x, y, z, λ) datacubes. Optical sectioning is implemented by structured illumination. The device's spectral imaging performance is demonstrated in a multispectral microsphere and mouse kidney tissue fluorescence imaging experiment. We also compare quantitatively the depth-resolved IMS with a hyperspectral confocal microscope (HCM) in a standard fluorescent bead imaging experiment. The comparison results show that despite the use of a light source with four orders of magnitude lower intensity in the IMS than that in the HCM, the image signal-to-noise ratio acquired by the IMS is 2.6 times higher than that achieved by the equivalent confocal approach.",

author = "Liang Gao and Noah Bedard and Nathan Hagen and Kester, {Robert T.} and Tkaczyk, {Tomasz S.}",

year = "2011",

month = aug,

day = "29",

doi = "10.1364/OE.19.017439",

language = "English (US)",

volume = "19",

pages = "17439--17452",

journal = "Optics Express",

issn = "1094-4087",

publisher = "The Optical Society",

number = "18",

}

TY - JOUR

T1 - Depth-resolved image mapping spectrometer (IMS) with structured illumination

AU - Gao, Liang

AU - Bedard, Noah

AU - Hagen, Nathan

AU - Kester, Robert T.

AU - Tkaczyk, Tomasz S.

PY - 2011/8/29

Y1 - 2011/8/29

N2 - We present a depth-resolved Image Mapping Spectrometer (IMS) which is capable of acquiring 4D (x, y, z, λ) datacubes. Optical sectioning is implemented by structured illumination. The device's spectral imaging performance is demonstrated in a multispectral microsphere and mouse kidney tissue fluorescence imaging experiment. We also compare quantitatively the depth-resolved IMS with a hyperspectral confocal microscope (HCM) in a standard fluorescent bead imaging experiment. The comparison results show that despite the use of a light source with four orders of magnitude lower intensity in the IMS than that in the HCM, the image signal-to-noise ratio acquired by the IMS is 2.6 times higher than that achieved by the equivalent confocal approach.

AB - We present a depth-resolved Image Mapping Spectrometer (IMS) which is capable of acquiring 4D (x, y, z, λ) datacubes. Optical sectioning is implemented by structured illumination. The device's spectral imaging performance is demonstrated in a multispectral microsphere and mouse kidney tissue fluorescence imaging experiment. We also compare quantitatively the depth-resolved IMS with a hyperspectral confocal microscope (HCM) in a standard fluorescent bead imaging experiment. The comparison results show that despite the use of a light source with four orders of magnitude lower intensity in the IMS than that in the HCM, the image signal-to-noise ratio acquired by the IMS is 2.6 times higher than that achieved by the equivalent confocal approach.

UR - http://www.scopus.com/inward/record.url?scp=80052214910&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80052214910&partnerID=8YFLogxK

U2 - 10.1364/OE.19.017439

DO - 10.1364/OE.19.017439

M3 - Article

C2 - 21935110

AN - SCOPUS:80052214910

VL - 19

SP - 17439

EP - 17452

JO - Optics Express

JF - Optics Express

SN - 1094-4087

IS - 18

ER -

Depth-resolved image mapping spectrometer (IMS) with structured illumination

Abstract

ASJC Scopus subject areas

Access to Document

Other links

Fingerprint

Cite this