TY - JOUR
T1 - Deproteinization of Cortical Bone
T2 - Effects of Different Treatments
AU - Su, Frances Y.
AU - Pang, Siyuan
AU - Ling, Yik Tung Tracy
AU - Shyu, Peter
AU - Novitskaya, Ekaterina
AU - Seo, Kyungah
AU - Lambert, Sofia
AU - Zarate, Kimberlin
AU - Graeve, Olivia A.
AU - Jasiuk, Iwona
AU - McKittrick, Joanna
N1 - Publisher Copyright:
© 2018, Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2018/11/1
Y1 - 2018/11/1
N2 - Bone is a biological composite material having collagen and mineral as its main constituents. In order to better understand the arrangement of the mineral phase in bone, porcine cortical bone was deproteinized using different chemical treatments. This study aims to determine the best method to remove the protein constituent while preserving the mineral component. Chemicals used were H2O2, NaOCl, NaOH, and KOH, and the efficacy of deproteinization treatments was determined by thermogravimetric analysis and Raman spectroscopy. The structure of the residual mineral parts was examined using scanning electron microscopy. X-ray diffraction was used to confirm that the mineral component was not altered by the chemical treatments. NaOCl was found to be the most effective method for deproteinization and the mineral phase was self-standing, supporting the hypothesis that bone is an interpenetrating composite. Thermogravimetric analyses and Raman spectroscopy results showed the preservation of mineral crystallinity and presence of residual organic material after all chemical treatments. A defatting step, which has not previously been used in conjunction with deproteinization to isolate the mineral phase, was also used. Finally, Raman spectroscopy demonstrated that the inclusion of a defatting procedure resulted in the removal of some but not all residual protein in the bone.
AB - Bone is a biological composite material having collagen and mineral as its main constituents. In order to better understand the arrangement of the mineral phase in bone, porcine cortical bone was deproteinized using different chemical treatments. This study aims to determine the best method to remove the protein constituent while preserving the mineral component. Chemicals used were H2O2, NaOCl, NaOH, and KOH, and the efficacy of deproteinization treatments was determined by thermogravimetric analysis and Raman spectroscopy. The structure of the residual mineral parts was examined using scanning electron microscopy. X-ray diffraction was used to confirm that the mineral component was not altered by the chemical treatments. NaOCl was found to be the most effective method for deproteinization and the mineral phase was self-standing, supporting the hypothesis that bone is an interpenetrating composite. Thermogravimetric analyses and Raman spectroscopy results showed the preservation of mineral crystallinity and presence of residual organic material after all chemical treatments. A defatting step, which has not previously been used in conjunction with deproteinization to isolate the mineral phase, was also used. Finally, Raman spectroscopy demonstrated that the inclusion of a defatting procedure resulted in the removal of some but not all residual protein in the bone.
KW - Cortical bone
KW - Deproteinization
KW - Raman spectroscopy
KW - Scanning electron microscopy
KW - Thermogravimetric analysis
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U2 - 10.1007/s00223-018-0453-x
DO - 10.1007/s00223-018-0453-x
M3 - Article
C2 - 30022228
AN - SCOPUS:85050248993
SN - 0171-967X
VL - 103
SP - 554
EP - 566
JO - Calcified Tissue International
JF - Calcified Tissue International
IS - 5
ER -