Deposition kinetics of bacteriophage MS2 to natural organic matter: Role of divalent cations

Mai Pham, Eric A. Mintz, Thanh H. Nguyen

Research output: Contribution to journalArticlepeer-review


The role of divalent cations (i.e. Ca2+ and Mg2+) in the deposition kinetics of the bacteriophage MS2 onto flat bare silica surfaces and Suwannee River Natural Organic Matter (SRNOM)-coated silica surfaces was investigated using a Quartz Crystal Microbalance (QCM) coupled with a radial stagnation point flow (RSPF) system. Experimental results demonstrated that attachment efficiencies of MS2 onto the SRNOM surface were seven to seventeen times higher in the presence of Ca2+ than in the presence of Mg2+. A similar trend was observed for the adsorption of polyglutamic acid, which is one of the carboxylate-containing amino acid residues found on the surface of MS2 capsids present on SRNOM-coated surfaces. The difference in attachment rates in a solution containing either Ca2+ or Mg2+ can be explained by a stronger tendency of Ca2+ compared to Mg2+ to form cation bridges by binding to carboxylate groups of both the SRNOM and the MS2 capsids. Moreover, higher attachment efficiencies of MS2 onto the SRNOM-coated silica surfaces compared to those on bare silica surfaces in the presence of either Ca2+ or Mg2+ at concentrations higher than 0.3 mM emphasized the important role of SRNOM carboxylate groups. Experimental data also showed reduced attachment efficiency of MS2 to SRNOM-coated surfaces in solution containing 1 mg/L SRNOM.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalJournal of Colloid And Interface Science
Issue number1
StatePublished - Oct 1 2009


  • Adsorption
  • Bacteriophage
  • Deposition
  • Divalent cations
  • Natural organic matter
  • Quartz crystal microbalance

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Surfaces, Coatings and Films
  • Colloid and Surface Chemistry


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