Deployment of regulatory genes during gastrulation and germ layer specification in a model spiralian mollusc Crepidula

Kimberly J. Perry, Deirdre C. Lyons, Marta Truchado-Garcia, Antje H.L. Fischer, Lily W. Helfrich, Kimberly B. Johansson, Julie C. Diamond, Cristina Grande, Jonathan Q. Henry

Research output: Contribution to journalArticlepeer-review

Abstract

Background: During gastrulation, endoderm and mesoderm are specified from a bipotential precursor (endomesoderm) that is argued to be homologous across bilaterians. Spiralians also generate mesoderm from ectodermal precursors (ectomesoderm), which arises near the blastopore. While a conserved gene regulatory network controls specification of endomesoderm in deuterostomes and ecdysozoans, little is known about genes controlling specification or behavior of either source of spiralian mesoderm or the digestive tract. Results: Using the mollusc Crepidula, we examined conserved regulatory factors and compared their expression to fate maps to score expression in the germ layers, blastopore lip, and digestive tract. Many genes were expressed in both ecto- and endomesoderm, but only five were expressed in ectomesoderm exclusively. The latter may contribute to epithelial-to-mesenchymal transition seen in ectomesoderm. Conclusions: We present the first comparison of genes expressed during spiralian gastrulation in the context of high-resolution fate maps. We found variation of genes expressed in the blastopore lip, mouth, and cells that will form the anus. Shared expression of many genes in both mesodermal sources suggests that components of the conserved endomesoderm program were either co-opted for ectomesoderm formation or that ecto- and endomesoderm are derived from a common mesodermal precursor that became subdivided into distinct domains during evolution.

Original languageEnglish (US)
Pages (from-to)1215-1248
Number of pages34
JournalDevelopmental Dynamics
Volume244
Issue number10
DOIs
StatePublished - Oct 1 2015

Keywords

  • Anus
  • Blastopore
  • EMT
  • Endoderm
  • Foregut
  • Gastrulation
  • Hindgut
  • Mesoderm
  • Mollusca
  • Mouth
  • Spiralia

ASJC Scopus subject areas

  • Developmental Biology

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