Deoxyribozymes: DNA catalysts for bioorganic chemistry

Research output: Contribution to journalReview article

Abstract

Deoxyribozymes are DNA molecules with catalytic activity. For historical and practical reasons, essentially all reported deoxyribozymes catalyze reactions of nucleic acid substrates, although this is probably not a fundamental limitation. In vitro selection strategies have been used to identify many deoxyribozymes that catalyze RNA cleavage, RNA and DNA ligation, and a variety of covalent modification reactions of nucleic acid substrates. Many deoxyribozymes are capable of catalysis with substantial rate enhancements reaching up to 1010-fold over background, and their very high selectivities would often be difficult or impossible to achieve using traditional organic synthesis approaches. This report summarizes the current utility and potential future applications of deoxyribozymes from the bioorganic chemistry perspective.

Original languageEnglish (US)
Pages (from-to)2701-2706
Number of pages6
JournalOrganic and Biomolecular Chemistry
Volume2
Issue number19
DOIs
StatePublished - Oct 7 2004

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Catalytic DNA
nucleic acids
deoxyribonucleic acid
chemistry
catalysts
Catalysts
DNA
catalysis
catalytic activity
cleavage
selectivity
Nucleic Acids
augmentation
RNA Cleavage
RNA
synthesis
Synthetic Chemistry Techniques
molecules
Substrates
Catalysis

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

Deoxyribozymes : DNA catalysts for bioorganic chemistry. / Silverman, Scott K.

In: Organic and Biomolecular Chemistry, Vol. 2, No. 19, 07.10.2004, p. 2701-2706.

Research output: Contribution to journalReview article

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