Deoxyribozyme-catalyzed labeling of RNA

Dana A. Baum, Scott K Silverman

Research output: Contribution to journalArticle

Abstract

Tag team: A deoxyribozyme is used in a general strategy for site-specific covalent modification of RNA. This strategy was used to append the fluorescence resonant energy transfer (FRET) pair, fluorescein and tetramethylrhodamine (tamra), to the 160-nucleotide P4-P6 RNA, thereby enabling a FRET experiment to be performed for this RNA. (Graph Presented).

Original languageEnglish (US)
Pages (from-to)3502-3504
Number of pages3
JournalAngewandte Chemie - International Edition
Volume46
Issue number19
DOIs
StatePublished - Jun 29 2007

Fingerprint

Catalytic DNA
RNA
Labeling
Energy transfer
Fluorescence
Nucleotides
Fluorescein
Experiments

Keywords

  • Deoxyribozymes
  • Fluorescent probes
  • Fret (fluorescence resonant energy transfer)
  • Macromolecular folding
  • RNA structures

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

Cite this

Deoxyribozyme-catalyzed labeling of RNA. / Baum, Dana A.; Silverman, Scott K.

In: Angewandte Chemie - International Edition, Vol. 46, No. 19, 29.06.2007, p. 3502-3504.

Research output: Contribution to journalArticle

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