Deoxyribozyme-catalyzed labeling of RNA

Dana A. Baum, Scott K. Silverman

Research output: Contribution to journalArticle

Abstract

Tag team: A deoxyribozyme is used in a general strategy for site-specific covalent modification of RNA. This strategy was used to append the fluorescence resonant energy transfer (FRET) pair, fluorescein and tetramethylrhodamine (tamra), to the 160-nucleotide P4-P6 RNA, thereby enabling a FRET experiment to be performed for this RNA. (Graph Presented).

Original languageEnglish (US)
Pages (from-to)3502-3504
Number of pages3
JournalAngewandte Chemie - International Edition
Volume46
Issue number19
DOIs
StatePublished - Jun 29 2007

Keywords

  • Deoxyribozymes
  • Fluorescent probes
  • Fret (fluorescence resonant energy transfer)
  • Macromolecular folding
  • RNA structures

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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