Abstract
It isworthwhile considering that only some 30 species make up the bulk of the bacterial population in human faeces at any one time based on the classical cultivation-based approach [7, 14]. The situation in the rumen is similar. Thus, it is practical to focus on specific groups of interest within the complex community. These may be the predominant or the most active species, specific physiological groups or readily identifiable (genetic) clusters of phylogenetically related organisms. Several 16S rDNA fingerprinting techniques can be invaluable for selecting and monitoring sequences or phylogenetic groups of interest and are described below.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Gut Microbial Ecology for Ruminants |
| Publisher | Springer |
| Pages | 119-128 |
| Number of pages | 10 |
| ISBN (Print) | 1402037902, 9781402037900 |
| DOIs | |
| State | Published - Dec 1 2005 |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
Cite this
Denaturing gradient gel electrophoresis. / Kocherginskaya, Svetlana A.; Cann, Isaac; Mackie, Roderick Ian.
Methods in Gut Microbial Ecology for Ruminants. Springer, 2005. p. 119-128.Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Denaturing gradient gel electrophoresis
AU - Kocherginskaya, Svetlana A.
AU - Cann, Isaac
AU - Mackie, Roderick Ian
PY - 2005/12/1
Y1 - 2005/12/1
N2 - It isworthwhile considering that only some 30 species make up the bulk of the bacterial population in human faeces at any one time based on the classical cultivation-based approach [7, 14]. The situation in the rumen is similar. Thus, it is practical to focus on specific groups of interest within the complex community. These may be the predominant or the most active species, specific physiological groups or readily identifiable (genetic) clusters of phylogenetically related organisms. Several 16S rDNA fingerprinting techniques can be invaluable for selecting and monitoring sequences or phylogenetic groups of interest and are described below.
AB - It isworthwhile considering that only some 30 species make up the bulk of the bacterial population in human faeces at any one time based on the classical cultivation-based approach [7, 14]. The situation in the rumen is similar. Thus, it is practical to focus on specific groups of interest within the complex community. These may be the predominant or the most active species, specific physiological groups or readily identifiable (genetic) clusters of phylogenetically related organisms. Several 16S rDNA fingerprinting techniques can be invaluable for selecting and monitoring sequences or phylogenetic groups of interest and are described below.
UR - http://www.scopus.com/inward/record.url?scp=34447125465&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34447125465&partnerID=8YFLogxK
U2 - 10.1007/1-4020-3791-0_9
DO - 10.1007/1-4020-3791-0_9
M3 - Chapter
AN - SCOPUS:34447125465
SN - 1402037902
SN - 9781402037900
SP - 119
EP - 128
BT - Methods in Gut Microbial Ecology for Ruminants
PB - Springer
ER -