Abstract
It isworthwhile considering that only some 30 species make up the bulk of the bacterial population in human faeces at any one time based on the classical cultivation-based approach [7, 14]. The situation in the rumen is similar. Thus, it is practical to focus on specific groups of interest within the complex community. These may be the predominant or the most active species, specific physiological groups or readily identifiable (genetic) clusters of phylogenetically related organisms. Several 16S rDNA fingerprinting techniques can be invaluable for selecting and monitoring sequences or phylogenetic groups of interest and are described below.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Gut Microbial Ecology for Ruminants |
| Publisher | Springer |
| Pages | 119-128 |
| Number of pages | 10 |
| ISBN (Print) | 1402037902, 9781402037900 |
| DOIs | |
| State | Published - Dec 1 2005 |
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
Fingerprint Dive into the research topics of 'Denaturing gradient gel electrophoresis'. Together they form a unique fingerprint.
Cite this
Kocherginskaya, S. A., Cann, I., & Mackie, R. I. (2005). Denaturing gradient gel electrophoresis. In Methods in Gut Microbial Ecology for Ruminants (pp. 119-128). Springer. https://doi.org/10.1007/1-4020-3791-0_9