Denaturing gradient gel electrophoresis

Svetlana A. Kocherginskaya; Isaac Cann; Roderick Ian Mackie

doi:10.1007/1-4020-3791-0_9

Denaturing gradient gel electrophoresis

Svetlana A. Kocherginskaya, Isaac Cann, Roderick Ian Mackie

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

It isworthwhile considering that only some 30 species make up the bulk of the bacterial population in human faeces at any one time based on the classical cultivation-based approach [7, 14]. The situation in the rumen is similar. Thus, it is practical to focus on specific groups of interest within the complex community. These may be the predominant or the most active species, specific physiological groups or readily identifiable (genetic) clusters of phylogenetically related organisms. Several 16S rDNA fingerprinting techniques can be invaluable for selecting and monitoring sequences or phylogenetic groups of interest and are described below.

Original language	English (US)
Title of host publication	Methods in Gut Microbial Ecology for Ruminants
Publisher	Springer
Pages	119-128
Number of pages	10
ISBN (Print)	1402037902, 9781402037900
DOIs	https://doi.org/10.1007/1-4020-3791-0_9
State	Published - Dec 1 2005

ASJC Scopus subject areas

Agricultural and Biological Sciences(all)

Access to Document

10.1007/1-4020-3791-0_9

Fingerprint Dive into the research topics of 'Denaturing gradient gel electrophoresis'. Together they form a unique fingerprint.

Cite this

@inbook{7a5032eca983407992be5e6c238bd839,

title = "Denaturing gradient gel electrophoresis",

abstract = "It isworthwhile considering that only some 30 species make up the bulk of the bacterial population in human faeces at any one time based on the classical cultivation-based approach [7, 14]. The situation in the rumen is similar. Thus, it is practical to focus on specific groups of interest within the complex community. These may be the predominant or the most active species, specific physiological groups or readily identifiable (genetic) clusters of phylogenetically related organisms. Several 16S rDNA fingerprinting techniques can be invaluable for selecting and monitoring sequences or phylogenetic groups of interest and are described below.",

author = "Kocherginskaya, {Svetlana A.} and Isaac Cann and Mackie, {Roderick Ian}",

year = "2005",

month = dec,

day = "1",

doi = "10.1007/1-4020-3791-0_9",

language = "English (US)",

isbn = "1402037902",

pages = "119--128",

booktitle = "Methods in Gut Microbial Ecology for Ruminants",

publisher = "Springer",

}

TY - CHAP

T1 - Denaturing gradient gel electrophoresis

AU - Kocherginskaya, Svetlana A.

AU - Cann, Isaac

AU - Mackie, Roderick Ian

PY - 2005/12/1

Y1 - 2005/12/1

N2 - It isworthwhile considering that only some 30 species make up the bulk of the bacterial population in human faeces at any one time based on the classical cultivation-based approach [7, 14]. The situation in the rumen is similar. Thus, it is practical to focus on specific groups of interest within the complex community. These may be the predominant or the most active species, specific physiological groups or readily identifiable (genetic) clusters of phylogenetically related organisms. Several 16S rDNA fingerprinting techniques can be invaluable for selecting and monitoring sequences or phylogenetic groups of interest and are described below.

AB - It isworthwhile considering that only some 30 species make up the bulk of the bacterial population in human faeces at any one time based on the classical cultivation-based approach [7, 14]. The situation in the rumen is similar. Thus, it is practical to focus on specific groups of interest within the complex community. These may be the predominant or the most active species, specific physiological groups or readily identifiable (genetic) clusters of phylogenetically related organisms. Several 16S rDNA fingerprinting techniques can be invaluable for selecting and monitoring sequences or phylogenetic groups of interest and are described below.

UR - http://www.scopus.com/inward/record.url?scp=34447125465&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34447125465&partnerID=8YFLogxK

U2 - 10.1007/1-4020-3791-0_9

DO - 10.1007/1-4020-3791-0_9

M3 - Chapter

AN - SCOPUS:34447125465

SN - 1402037902

SN - 9781402037900

SP - 119

EP - 128

BT - Methods in Gut Microbial Ecology for Ruminants

PB - Springer

ER -