Denaturing gradient gel electrophoresis

Svetlana A. Kocherginskaya; Isaac Cann; Roderick Ian Mackie

doi:10.1007/1-4020-3791-0_9

Denaturing gradient gel electrophoresis

Svetlana A. Kocherginskaya, Isaac Cann, Roderick Ian Mackie

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

It isworthwhile considering that only some 30 species make up the bulk of the bacterial population in human faeces at any one time based on the classical cultivation-based approach [7, 14]. The situation in the rumen is similar. Thus, it is practical to focus on specific groups of interest within the complex community. These may be the predominant or the most active species, specific physiological groups or readily identifiable (genetic) clusters of phylogenetically related organisms. Several 16S rDNA fingerprinting techniques can be invaluable for selecting and monitoring sequences or phylogenetic groups of interest and are described below.

Original language	English (US)
Title of host publication	Methods in Gut Microbial Ecology for Ruminants
Publisher	Springer
Pages	119-128
Number of pages	10
ISBN (Print)	1402037902, 9781402037900
DOIs	https://doi.org/10.1007/1-4020-3791-0_9
State	Published - Dec 1 2005

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ASJC Scopus subject areas

Agricultural and Biological Sciences(all)

Cite this

Kocherginskaya, S. A., Cann, I., & Mackie, R. I. (2005). Denaturing gradient gel electrophoresis. In Methods in Gut Microbial Ecology for Ruminants (pp. 119-128). Springer. https://doi.org/10.1007/1-4020-3791-0_9

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10.1007/1-4020-3791-0_9