Deletion of the gene encoding cytochrome b562 from Rhodobacter sphaeroides

Chang Hyon Yun; Blanca Barquera; Koh Iba; Ken ichiro Takamiya; James Shapleigh; Antony R. Crofts; Robert B. Gennis

doi:10.1111/j.1574-6968.1994.tb07015.x

Deletion of the gene encoding cytochrome b₅₆₂ from Rhodobacter sphaeroides

Chang Hyon Yun, Blanca Barquera, Koh Iba, Ken ichiro Takamiya, James Shapleigh, Antony R. Crofts, Robert B. Gennis

Biochemistry

Research output: Contribution to journal › Article › peer-review

Abstract

Cytochrome b₅₆₂ is a membrane bound di-heme cytochrome previously isolated and characterized from the membranes of photosynthetically grown Rhodobacter sphaeroides. Although originally suggested to be a component of the bc₁ complex, subsequent work, including the sequence of the gene encoding the protein, indicates that this is not the case. More recently, it has been proposed to be a component of complex II (succinate-ubiquinol oxidoreductase). In an effort to determine the function of this cytochrome, the gene encoding the protein has been deleted from the chromosome, and the resulting strain has been examined. The results show that this cytochrome is not as essential component of complex II, the bc₁ complex, or any of the respiratory oxidases.

Original language	English (US)
Pages (from-to)	105-110
Number of pages	6
Journal	FEMS microbiology letters
Volume	120
Issue number	1-2
DOIs	https://doi.org/10.1111/j.1574-6968.1994.tb07015.x
State	Published - Jul 1 1994

Keywords

Cytochrome
Gene deletion
Rhodobacter sphaeroides

ASJC Scopus subject areas

General Medicine

Online availability

10.1111/j.1574-6968.1994.tb07015.x

Library availability

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Cite this

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title = "Deletion of the gene encoding cytochrome b562 from Rhodobacter sphaeroides",

abstract = "Cytochrome b562 is a membrane bound di-heme cytochrome previously isolated and characterized from the membranes of photosynthetically grown Rhodobacter sphaeroides. Although originally suggested to be a component of the bc1 complex, subsequent work, including the sequence of the gene encoding the protein, indicates that this is not the case. More recently, it has been proposed to be a component of complex II (succinate-ubiquinol oxidoreductase). In an effort to determine the function of this cytochrome, the gene encoding the protein has been deleted from the chromosome, and the resulting strain has been examined. The results show that this cytochrome is not as essential component of complex II, the bc1 complex, or any of the respiratory oxidases.",

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AU - Yun, Chang Hyon

AU - Barquera, Blanca

AU - Iba, Koh

AU - Takamiya, Ken ichiro

AU - Shapleigh, James

AU - Crofts, Antony R.

AU - Gennis, Robert B.

PY - 1994/7/1

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AB - Cytochrome b562 is a membrane bound di-heme cytochrome previously isolated and characterized from the membranes of photosynthetically grown Rhodobacter sphaeroides. Although originally suggested to be a component of the bc1 complex, subsequent work, including the sequence of the gene encoding the protein, indicates that this is not the case. More recently, it has been proposed to be a component of complex II (succinate-ubiquinol oxidoreductase). In an effort to determine the function of this cytochrome, the gene encoding the protein has been deleted from the chromosome, and the resulting strain has been examined. The results show that this cytochrome is not as essential component of complex II, the bc1 complex, or any of the respiratory oxidases.

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