Deletion of the gene encoding cytochrome b562 from Rhodobacter sphaeroides

Chang Hyon Yun, Blanca Barquera, Koh Iba, Ken ichiro Takamiya, James Shapleigh, Antony R. Crofts, Robert B. Gennis

Research output: Contribution to journalArticlepeer-review

Abstract

Cytochrome b562 is a membrane bound di-heme cytochrome previously isolated and characterized from the membranes of photosynthetically grown Rhodobacter sphaeroides. Although originally suggested to be a component of the bc1 complex, subsequent work, including the sequence of the gene encoding the protein, indicates that this is not the case. More recently, it has been proposed to be a component of complex II (succinate-ubiquinol oxidoreductase). In an effort to determine the function of this cytochrome, the gene encoding the protein has been deleted from the chromosome, and the resulting strain has been examined. The results show that this cytochrome is not as essential component of complex II, the bc1 complex, or any of the respiratory oxidases.

Original languageEnglish (US)
Pages (from-to)105-110
Number of pages6
JournalFEMS microbiology letters
Volume120
Issue number1-2
DOIs
StatePublished - Jul 1 1994

Keywords

  • Cytochrome
  • Gene deletion
  • Rhodobacter sphaeroides

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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