Deletion of ALS5, ALS6 or ALS7 increases adhesion of Candida albicans to human vascular endothelial and buccal epithelial cells

Xiaomin Zhao; Soon Hwan Oh; Lois L. Hoyer

doi:10.1080/13693780701377162

Deletion of ALS5, ALS6 or ALS7 increases adhesion of Candida albicans to human vascular endothelial and buccal epithelial cells

Xiaomin Zhao, Soon Hwan Oh, Lois L. Hoyer

Research output: Contribution to journal › Article › peer-review

Abstract

Candida albicans yeast forms deleted for ALS5, ALS6 or ALS7 are more adherent than a relevant control strain to human vascular endothelial cell monolayers and buccal epithelial cells. In the buccal and vaginal reconstituted human epithelium (RHE) disease models, however, mutant and control strains caused a similar degree of tissue destruction. Deletion of ALS5 or ALS6 significantly slowed growth of the mutant strain; this phenotype was not affected by addition of excess uridine to the culture medium. These studies demonstrate similar phenotypic characteristics for the als5Δ/als5Δ, als6Δ/als6Δ and als7Δ/ als7Δ strains that are not observed in any of the other C. albicans alsΔ/alsΔ isolates.

Original language	English (US)
Pages (from-to)	429-434
Number of pages	6
Journal	Medical Mycology
Volume	45
Issue number	5
DOIs	https://doi.org/10.1080/13693780701377162
State	Published - Aug 2007

Keywords

ALS gene family
Adhesion
Candida albicans
Host-pathogen interaction

ASJC Scopus subject areas

Infectious Diseases

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title = "Deletion of ALS5, ALS6 or ALS7 increases adhesion of Candida albicans to human vascular endothelial and buccal epithelial cells",

abstract = "Candida albicans yeast forms deleted for ALS5, ALS6 or ALS7 are more adherent than a relevant control strain to human vascular endothelial cell monolayers and buccal epithelial cells. In the buccal and vaginal reconstituted human epithelium (RHE) disease models, however, mutant and control strains caused a similar degree of tissue destruction. Deletion of ALS5 or ALS6 significantly slowed growth of the mutant strain; this phenotype was not affected by addition of excess uridine to the culture medium. These studies demonstrate similar phenotypic characteristics for the als5Δ/als5Δ, als6Δ/als6Δ and als7Δ/ als7Δ strains that are not observed in any of the other C. albicans alsΔ/alsΔ isolates.",

keywords = "ALS gene family, Adhesion, Candida albicans, Host-pathogen interaction",

author = "Xiaomin Zhao and Oh, {Soon Hwan} and Hoyer, {Lois L.}",

note = "Funding Information: This research was funded by grant DE14158 from the National Institute of Dental and Craniofacial Research, National Institutes of Health. This investigation was conducted in a facility constructed with support from Research Facilities Improvement Program Grant Number C06 RR16515·01 from the National Center for Research Resources, National Institutes of Health.",

year = "2007",

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doi = "10.1080/13693780701377162",

language = "English (US)",

volume = "45",

pages = "429--434",

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AU - Zhao, Xiaomin

AU - Oh, Soon Hwan

AU - Hoyer, Lois L.

N1 - Funding Information: This research was funded by grant DE14158 from the National Institute of Dental and Craniofacial Research, National Institutes of Health. This investigation was conducted in a facility constructed with support from Research Facilities Improvement Program Grant Number C06 RR16515·01 from the National Center for Research Resources, National Institutes of Health.

PY - 2007/8

Y1 - 2007/8

N2 - Candida albicans yeast forms deleted for ALS5, ALS6 or ALS7 are more adherent than a relevant control strain to human vascular endothelial cell monolayers and buccal epithelial cells. In the buccal and vaginal reconstituted human epithelium (RHE) disease models, however, mutant and control strains caused a similar degree of tissue destruction. Deletion of ALS5 or ALS6 significantly slowed growth of the mutant strain; this phenotype was not affected by addition of excess uridine to the culture medium. These studies demonstrate similar phenotypic characteristics for the als5Δ/als5Δ, als6Δ/als6Δ and als7Δ/ als7Δ strains that are not observed in any of the other C. albicans alsΔ/alsΔ isolates.

AB - Candida albicans yeast forms deleted for ALS5, ALS6 or ALS7 are more adherent than a relevant control strain to human vascular endothelial cell monolayers and buccal epithelial cells. In the buccal and vaginal reconstituted human epithelium (RHE) disease models, however, mutant and control strains caused a similar degree of tissue destruction. Deletion of ALS5 or ALS6 significantly slowed growth of the mutant strain; this phenotype was not affected by addition of excess uridine to the culture medium. These studies demonstrate similar phenotypic characteristics for the als5Δ/als5Δ, als6Δ/als6Δ and als7Δ/ als7Δ strains that are not observed in any of the other C. albicans alsΔ/alsΔ isolates.

KW - ALS gene family

KW - Adhesion

KW - Candida albicans

KW - Host-pathogen interaction

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SN - 1369-3786

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Deletion of ALS5, ALS6 or ALS7 increases adhesion of Candida albicans to human vascular endothelial and buccal epithelial cells

Abstract

Keywords

ASJC Scopus subject areas

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