TY - JOUR
T1 - Degradation of complex arabinoxylans by human colonic Bacteroidetes
AU - Pereira, Gabriel V.
AU - Abdel-Hamid, Ahmed M.
AU - Dutta, Soumajit
AU - D’Alessandro-Gabazza, Corina N.
AU - Wefers, Daniel
AU - Farris, Jacob A.
AU - Bajaj, Shiv
AU - Wawrzak, Zdzislaw
AU - Atomi, Haruyuki
AU - Mackie, Roderick I.
AU - Gabazza, Esteban C.
AU - Shukla, Diwakar
AU - Koropatkin, Nicole M.
AU - Cann, Isaac
N1 - Funding Information:
The research was supported by the National Institute of General Medical Sciences of the National Institutes of Health under Award Number RO1GM140306 to R.I.M., N.M.K., and I.C., and in part by funding from the Microbiome Metabolic Engineering Theme (MME) of the Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana Champaign. The funders had no role in the study design, data analyses, decision to publish, or preparation of the manuscript. I.C. would like to acknowledge support during sabbatical by the Top Global University Program, Kyoto University, Japan.
PY - 2021/12
Y1 - 2021/12
N2 - Some Bacteroidetes and other human colonic bacteria can degrade arabinoxylans, common polysaccharides found in dietary fiber. Previous work has identified gene clusters (polysaccharide-utilization loci, PULs) for degradation of simple arabinoxylans. However, the degradation of complex arabinoxylans (containing side chains such as ferulic acid, a phenolic compound) is poorly understood. Here, we identify a PUL that encodes multiple esterases for degradation of complex arabinoxylans in Bacteroides species. The PUL is specifically upregulated in the presence of complex arabinoxylans. We characterize some of the esterases biochemically and structurally, and show that they release ferulic acid from complex arabinoxylans. Growth of four different colonic Bacteroidetes members, including Bacteroides intestinalis, on complex arabinoxylans results in accumulation of ferulic acid, a compound known to have antioxidative and immunomodulatory properties.
AB - Some Bacteroidetes and other human colonic bacteria can degrade arabinoxylans, common polysaccharides found in dietary fiber. Previous work has identified gene clusters (polysaccharide-utilization loci, PULs) for degradation of simple arabinoxylans. However, the degradation of complex arabinoxylans (containing side chains such as ferulic acid, a phenolic compound) is poorly understood. Here, we identify a PUL that encodes multiple esterases for degradation of complex arabinoxylans in Bacteroides species. The PUL is specifically upregulated in the presence of complex arabinoxylans. We characterize some of the esterases biochemically and structurally, and show that they release ferulic acid from complex arabinoxylans. Growth of four different colonic Bacteroidetes members, including Bacteroides intestinalis, on complex arabinoxylans results in accumulation of ferulic acid, a compound known to have antioxidative and immunomodulatory properties.
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U2 - 10.1038/s41467-020-20737-5
DO - 10.1038/s41467-020-20737-5
M3 - Article
C2 - 33469030
VL - 12
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
IS - 1
M1 - 459
ER -