Deep line-temporal focusing with high axial resolution and a large field-of-view using intracavity control and incoherent pulse shaping

Kai Lou, Bo Wang, Ah Young Jee, Steve Granick, François Amblard

Research output: Contribution to journalArticlepeer-review

Abstract

Line-temporal focusing has been recognized as an elegant strategy that provides two-photon microscopy with an effective means for fast imaging through parallelization, together with an improved resilience to scattering for deep imaging. However, the axial resolution remains sub-optimal, except when using high NA objectives and a small field-of-view. With the introduction of an intracavity control of the spectral width of the femtosecond laser to adaptively fill the back aperture of the objective lens, line-temporal focusing two-photon microscopy is demonstrated to reach near-diffraction-limited axial resolution with a large backaperture objective lens, and improved immunity to sample scattering. In addition, a new incoherent flattop beam shaping method is proposed which provides a uniform contrast with little degradation of the axial resolution along the focus line, even deep in the sample. This is demonstrated in large volumetric imaging of mouse lung samples.

Original languageEnglish (US)
Pages (from-to)4919-4922
Number of pages4
JournalOptics Letters
Volume43
Issue number20
DOIs
StatePublished - Oct 15 2018
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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