TY - JOUR
T1 - Decreased protein synthesis by polysomes, tRNA and aminoacyl-tRNA synthetases isolated from senescent rat liver
AU - Cook, J. R.
AU - Buetow, D. E.
N1 - Funding Information:
This research was supported in part by NIH grant GM 19641. J.R.C. was a trainee supported by NIH grant 07143. Data presented are from a thesis submitted by J.R.C. in partial fulfillment of the requirements for the Ph.D. degree at the University of Illinois, Urbana. We thank Carl Gilbert for the 3sS-labeled protein used in the protease assays.
PY - 1981/9
Y1 - 1981/9
N2 - A cell-free in vitro protein-synthesizing system isolated from adult (10-13 month) and senescent (24-30 month) female Wistar rat liver is described. Optimal concentrations of polysomes, aminoacyl-tRNA synthetases, tRNA, Mg2+, K+, and labeled amino acids are reported for this in vitro system. Both the rate and extent of protein synthesis are lower with the system from senescent liver compared to that from adult liver. Senescent polysomes, tRNA synthetases, and tRNA all contribute to this decrease. Free adult or senescent polysomes support protein synthesis to the same extent. However, compared to adult polysomes, senescent membrane-bound polysomes support less protein synthesis and account for an increased proportion of the total polysome fraction. Ribonuclease and protease activities are low in the adult and the senescent in vitro systems and do not account for the decreased protein synthesis with the latter.
AB - A cell-free in vitro protein-synthesizing system isolated from adult (10-13 month) and senescent (24-30 month) female Wistar rat liver is described. Optimal concentrations of polysomes, aminoacyl-tRNA synthetases, tRNA, Mg2+, K+, and labeled amino acids are reported for this in vitro system. Both the rate and extent of protein synthesis are lower with the system from senescent liver compared to that from adult liver. Senescent polysomes, tRNA synthetases, and tRNA all contribute to this decrease. Free adult or senescent polysomes support protein synthesis to the same extent. However, compared to adult polysomes, senescent membrane-bound polysomes support less protein synthesis and account for an increased proportion of the total polysome fraction. Ribonuclease and protease activities are low in the adult and the senescent in vitro systems and do not account for the decreased protein synthesis with the latter.
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U2 - 10.1016/0047-6374(81)90127-5
DO - 10.1016/0047-6374(81)90127-5
M3 - Article
C2 - 7311618
AN - SCOPUS:0019775307
SN - 0047-6374
VL - 17
SP - 41
EP - 52
JO - Mechanisms of Ageing and Development
JF - Mechanisms of Ageing and Development
IS - 1
ER -