TY - JOUR
T1 - DDT resistance in Drosophila correlates with Cyp6g1 over-expression and confers cross-resistance to the neonicotinoid imidacloprid
AU - Daborn, P.
AU - Boundy, S.
AU - Yen, J.
AU - Pittendrigh, B.
AU - Ffrench-Constant, R.
N1 - Funding Information:
Acknowledgements We thank M. Kreitman for ideas on the mapping of DDT resistance and in whose laboratory B.P. performed initial mapping studies on Wis1. We are also indebted to R.T. Roush for first drawing our attention to this gene whilst R. ff-C was a post doctoral fellow in his laboratory at Cornell University. S.B. was supported by a BBSRC CASE Studentship between the University of Bath and Syngenta co-ordinated by Robert Lind. R. ff-C is s upported by a Royal Society Merit Award. This paper is dedicated to Jim Crow for his initial work on mapping DDT resistance.
PY - 2001
Y1 - 2001
N2 - Mutagenesis can be used as a means of predicting likely mechanisms of resistance to novel classes of insecticides. We used chemical mutagenesis in Drosophila to screen for mutants that had become resistant to imidacloprid, a neonicotinoid insecticide. Here we report the isolation of two new dominant imidacloprid-resistant mutants. By recombinational mapping we show that these map to the same location as Rst(2)DDT. Furthermore, we show that pre-existing Rst(2)DDT alleles in turn confer cross-resistance to imidacloprid. In order to localize the Rst(2)DDT gene more precisely, we mapped resistance to both DDT and imidacloprid with respect to P-element markers whose genomic location is known. By screening for recombinants between these P-elements and resistance we localized the gene between 48D5-6 and 48F3-6 on the polytene chromosome map. The genomic sequence in this interval shows a cluster of cytochrome P450 genes, one of which, Cyp6g1, is over-expressed in all resistant strains examined. We are now testing the hypothesis that resistance to both compounds is associated with over-expression of this P450 gene.
AB - Mutagenesis can be used as a means of predicting likely mechanisms of resistance to novel classes of insecticides. We used chemical mutagenesis in Drosophila to screen for mutants that had become resistant to imidacloprid, a neonicotinoid insecticide. Here we report the isolation of two new dominant imidacloprid-resistant mutants. By recombinational mapping we show that these map to the same location as Rst(2)DDT. Furthermore, we show that pre-existing Rst(2)DDT alleles in turn confer cross-resistance to imidacloprid. In order to localize the Rst(2)DDT gene more precisely, we mapped resistance to both DDT and imidacloprid with respect to P-element markers whose genomic location is known. By screening for recombinants between these P-elements and resistance we localized the gene between 48D5-6 and 48F3-6 on the polytene chromosome map. The genomic sequence in this interval shows a cluster of cytochrome P450 genes, one of which, Cyp6g1, is over-expressed in all resistant strains examined. We are now testing the hypothesis that resistance to both compounds is associated with over-expression of this P450 gene.
KW - Cytochrome P450
KW - DDT
KW - Drosophila melanogaster
KW - Imidacloprid
KW - Insecticide resistance
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U2 - 10.1007/s004380100531
DO - 10.1007/s004380100531
M3 - Article
C2 - 11810226
AN - SCOPUS:0035207207
SN - 1617-4615
VL - 266
SP - 556
EP - 563
JO - Molecular Genetics and Genomics
JF - Molecular Genetics and Genomics
IS - 4
ER -