TY - JOUR
T1 - Dauer-specific dendrite arborization in C. elegans is regulated by KPC-1/furin
AU - Schroeder, Nathan E.
AU - Androwski, Rebecca J.
AU - Rashid, Alina
AU - Lee, Harksun
AU - Lee, Junho
AU - Barr, Maureen M.
N1 - Funding Information:
We thank the CGC, funded by the NIH Office of Research Infrastructure Programs (P40 OD010440), S. Shaham, T. Lamitina, M. Chalfie, and P. Swoboda for providing strains; Noriko Goldsmith for assistance with confocal microscopy; Natalia Morsci and members of the Barr lab for unpublished data and critical reading of the manuscript; C. Britt Carlson and Michael Klaszky for technical assistance; and David Hall for valuable advice. This work was funded by the USDA (2010-65106-20587) and the New Jersey Commission on Spinal Cord Research (CSCR12FEL004) to N.E.S. and the NIH (5R01DK59418) to MMB.
PY - 2013/8/19
Y1 - 2013/8/19
N2 - Background Dendrites often display remarkably complex and diverse morphologies that are influenced by developmental and environmental cues. Neuroplasticity in response to adverse environmental conditions entails both hypertrophy and resorption of dendrites. How dendrites rapidly alter morphology in response to unfavorable environmental conditions is unclear. The nematode Caenorhabditis elegans enters into a stress-resistant dauer larval stage in response to an adverse environment. Results Here we show that the IL2 bipolar sensory neurons undergo dendrite arborization and axon remodeling during dauer development. When dauer larvae are returned to favorable environmental conditions, animals resume reproductive development and IL2 dendritic branches retract, leaving behind remnant branches in postdauer L4 and adult animals. The C. elegans furin homolog KPC-1 is required for dauer IL2 dendritic arborization and dauer-specific nictation behavior. KPC-1 is also necessary for dendritic arborization of PVD and FLP sensory neurons. In mammals, furin is essential, ubiquitously expressed, and associated with numerous pathologies, including neurodegenerative diseases. While broadly expressed in C. elegans neurons and epithelia, KPC-1 acts cell autonomously in IL2 neurons to regulate dauer-specific dendritic arborization and nictation. Conclusions Neuroplasticity of the C. elegans IL2 sensory neurons provides a paradigm to study stress-induced and reversible dendritic branching, and the role of environmental and developmental cues in this process. The newly discovered role of KPC-1 in dendrite morphogenesis provides insight into the function of proprotein convertases in nervous system development.
AB - Background Dendrites often display remarkably complex and diverse morphologies that are influenced by developmental and environmental cues. Neuroplasticity in response to adverse environmental conditions entails both hypertrophy and resorption of dendrites. How dendrites rapidly alter morphology in response to unfavorable environmental conditions is unclear. The nematode Caenorhabditis elegans enters into a stress-resistant dauer larval stage in response to an adverse environment. Results Here we show that the IL2 bipolar sensory neurons undergo dendrite arborization and axon remodeling during dauer development. When dauer larvae are returned to favorable environmental conditions, animals resume reproductive development and IL2 dendritic branches retract, leaving behind remnant branches in postdauer L4 and adult animals. The C. elegans furin homolog KPC-1 is required for dauer IL2 dendritic arborization and dauer-specific nictation behavior. KPC-1 is also necessary for dendritic arborization of PVD and FLP sensory neurons. In mammals, furin is essential, ubiquitously expressed, and associated with numerous pathologies, including neurodegenerative diseases. While broadly expressed in C. elegans neurons and epithelia, KPC-1 acts cell autonomously in IL2 neurons to regulate dauer-specific dendritic arborization and nictation. Conclusions Neuroplasticity of the C. elegans IL2 sensory neurons provides a paradigm to study stress-induced and reversible dendritic branching, and the role of environmental and developmental cues in this process. The newly discovered role of KPC-1 in dendrite morphogenesis provides insight into the function of proprotein convertases in nervous system development.
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U2 - 10.1016/j.cub.2013.06.058
DO - 10.1016/j.cub.2013.06.058
M3 - Article
C2 - 23932402
AN - SCOPUS:84882601839
SN - 0960-9822
VL - 23
SP - 1527
EP - 1535
JO - Current Biology
JF - Current Biology
IS - 16
ER -