Data Analysis Methods for Fluorescence Imaging with One-Nanometer Accuracy (FIONA)

Paul R Selvin, Tyler Lougheed, Melinda Tonks Hoffman, Hyokeun Park, Hamza Balci, Benjamin H Blehm, Erdal Toprak

Research output: Contribution to journalArticlepeer-review

Abstract

INTRODUCTION: FIONA, short for fluorescence imaging with one-nanometer accuracy, is a simple method for achieving localization of single (or single groups of) fluorophores with nanometer accuracy in the xy plane. Data analysis of a FIONA experiment requires the use of several software programs. Data must be acquired and exported into a proper format before analysis can take place. This article describes various options for data analysis.

Original languageEnglish (US)
Pages (from-to)pdb.ip46
JournalCold Spring Harbor Protocols
Volume2007
DOIs
StatePublished - Oct 1 2007

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