Abstract

Relatively high levels of d-alanine (d-Ala), an endogenous d-amino acid, have been found in the endocrine systems of several animals, especially in the anterior pituitary; however, its functional importance remains largely unknown. We observed d-Ala in islets of Langerhans isolated from rat pancreas in significantly higher levels than in the anterior/intermediate pituitary; specifically, 180 ± 60 fmol d-Ala per islet (300 ± 100 nmol/g islet), and 10 ± 2.5 nmol/g of wet tissue in pituitary. Additionally, 12 ± 5% of the free Ala in the islets was in the d form, almost an order of magnitude higher than the percentage of d-Ala found in the pituitary. Surprisingly, glucose stimulation of the islets resulted in d-Ala release of 0.6 ± 0.5 fmol per islet. As d-Ala is stored in islets and released in response to changes in extracellular glucose, d-Ala may have a hormonal role.

Original languageEnglish (US)
Pages (from-to)328-333
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume447
Issue number2
DOIs
StatePublished - May 2 2014

Fingerprint

Islets of Langerhans
Alanine
Rats
Pancreas
Glucose
Endocrine System
Animals
Tissue
Amino Acids

Keywords

  • Capillary electrophoresis
  • Chiral separation
  • d-Alanine
  • d-Amino acid
  • Endocrine system
  • Islets of Langerhans

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

D-Alanine in the islets of Langerhans of rat pancreas. / Ota, Nobutoshi; Rubakhin, Stanislav; Sweedler, Jonathan V.

In: Biochemical and Biophysical Research Communications, Vol. 447, No. 2, 02.05.2014, p. 328-333.

Research output: Contribution to journalArticle

@article{7e41009b22554d9d948c90ed298cf315,
title = "D-Alanine in the islets of Langerhans of rat pancreas",
abstract = "Relatively high levels of d-alanine (d-Ala), an endogenous d-amino acid, have been found in the endocrine systems of several animals, especially in the anterior pituitary; however, its functional importance remains largely unknown. We observed d-Ala in islets of Langerhans isolated from rat pancreas in significantly higher levels than in the anterior/intermediate pituitary; specifically, 180 ± 60 fmol d-Ala per islet (300 ± 100 nmol/g islet), and 10 ± 2.5 nmol/g of wet tissue in pituitary. Additionally, 12 ± 5{\%} of the free Ala in the islets was in the d form, almost an order of magnitude higher than the percentage of d-Ala found in the pituitary. Surprisingly, glucose stimulation of the islets resulted in d-Ala release of 0.6 ± 0.5 fmol per islet. As d-Ala is stored in islets and released in response to changes in extracellular glucose, d-Ala may have a hormonal role.",
keywords = "Capillary electrophoresis, Chiral separation, d-Alanine, d-Amino acid, Endocrine system, Islets of Langerhans",
author = "Nobutoshi Ota and Stanislav Rubakhin and Sweedler, {Jonathan V}",
year = "2014",
month = "5",
day = "2",
doi = "10.1016/j.bbrc.2014.03.153",
language = "English (US)",
volume = "447",
pages = "328--333",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - D-Alanine in the islets of Langerhans of rat pancreas

AU - Ota, Nobutoshi

AU - Rubakhin, Stanislav

AU - Sweedler, Jonathan V

PY - 2014/5/2

Y1 - 2014/5/2

N2 - Relatively high levels of d-alanine (d-Ala), an endogenous d-amino acid, have been found in the endocrine systems of several animals, especially in the anterior pituitary; however, its functional importance remains largely unknown. We observed d-Ala in islets of Langerhans isolated from rat pancreas in significantly higher levels than in the anterior/intermediate pituitary; specifically, 180 ± 60 fmol d-Ala per islet (300 ± 100 nmol/g islet), and 10 ± 2.5 nmol/g of wet tissue in pituitary. Additionally, 12 ± 5% of the free Ala in the islets was in the d form, almost an order of magnitude higher than the percentage of d-Ala found in the pituitary. Surprisingly, glucose stimulation of the islets resulted in d-Ala release of 0.6 ± 0.5 fmol per islet. As d-Ala is stored in islets and released in response to changes in extracellular glucose, d-Ala may have a hormonal role.

AB - Relatively high levels of d-alanine (d-Ala), an endogenous d-amino acid, have been found in the endocrine systems of several animals, especially in the anterior pituitary; however, its functional importance remains largely unknown. We observed d-Ala in islets of Langerhans isolated from rat pancreas in significantly higher levels than in the anterior/intermediate pituitary; specifically, 180 ± 60 fmol d-Ala per islet (300 ± 100 nmol/g islet), and 10 ± 2.5 nmol/g of wet tissue in pituitary. Additionally, 12 ± 5% of the free Ala in the islets was in the d form, almost an order of magnitude higher than the percentage of d-Ala found in the pituitary. Surprisingly, glucose stimulation of the islets resulted in d-Ala release of 0.6 ± 0.5 fmol per islet. As d-Ala is stored in islets and released in response to changes in extracellular glucose, d-Ala may have a hormonal role.

KW - Capillary electrophoresis

KW - Chiral separation

KW - d-Alanine

KW - d-Amino acid

KW - Endocrine system

KW - Islets of Langerhans

UR - http://www.scopus.com/inward/record.url?scp=84899949420&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84899949420&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2014.03.153

DO - 10.1016/j.bbrc.2014.03.153

M3 - Article

C2 - 24721429

AN - SCOPUS:84899949420

VL - 447

SP - 328

EP - 333

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -