Cytoskeletal networks in an epithelial sheet: Mechanical characterization using composite diaphragm inflation

John C. Selby; Stephen A. Boppart; Mark A. Shannon

doi:10.1109/MMB.2005.1548425

Cytoskeletal networks in an epithelial sheet: Mechanical characterization using composite diaphragm inflation

John C. Selby, Stephen A. Boppart, Mark A. Shannon

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

A new experimental methodology combining concepts of classical mechanical testing with basic microfabrication techniques has been developed to investigate the mechanical architecture of cytoskeletal networks and anchoring junctions within a monolayer of normal human epidermal keratinocytes (NHEKs) before, during, and after the in vitro formation of an epithelial sheet. Referred to as composite diaphragm inflation (CDI), the method resembles a microscale model of a classical axisvmmetric biaxial membrane inflation experiment. CDI utilizes a free-standing polydimethylsiloxane (PDMS) elastomer membrane as a substrate for the culture of NHEKs into an epithelial sheet via calcium induction of cytoskeletal network reorganization and anchoring junction assembly. Together, the NHEK monolayer and the PDMS membrane form a composite diaphragm suitable for mechanical measurements. If the geometry and elastic properties of the PDMS membrane are scaled appropriately, then the prestress and apparent stiffness of the NHEK layer can be characterized by measured deviations in the load-displacement behavior of the composite diaphragm with respect to the behavior of the PDMS membrane measured prior to cell culture.

Original language	English (US)
Title of host publication	2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology
Pages	198-200
Number of pages	3
DOIs	https://doi.org/10.1109/MMB.2005.1548425
State	Published - 2005
Event	2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology - Oahu, HI, United States Duration: May 12 2005 → May 15 2005

Publication series

Name	2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology
Volume	2005

Other

Other	2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology
Country/Territory	United States
City	Oahu, HI
Period	5/12/05 → 5/15/05

Keywords

Anchoring junctions
Cell mechanics
Cytoskeleton

ASJC Scopus subject areas

General Engineering

Online availability

10.1109/MMB.2005.1548425

Library availability

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Cite this

Selby, J. C., Boppart, S. A., & Shannon, M. A. (2005). Cytoskeletal networks in an epithelial sheet: Mechanical characterization using composite diaphragm inflation. In 2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology (pp. 198-200). Article 1548425 (2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology; Vol. 2005). https://doi.org/10.1109/MMB.2005.1548425

Cytoskeletal networks in an epithelial sheet: Mechanical characterization using composite diaphragm inflation. / Selby, John C.; Boppart, Stephen A.; Shannon, Mark A.
2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology. 2005. p. 198-200 1548425 (2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology; Vol. 2005).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Selby, JC, Boppart, SA & Shannon, MA 2005, Cytoskeletal networks in an epithelial sheet: Mechanical characterization using composite diaphragm inflation. in 2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology., 1548425, 2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology, vol. 2005, pp. 198-200, 2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology, Oahu, HI, United States, 5/12/05. https://doi.org/10.1109/MMB.2005.1548425

Selby JC, Boppart SA, Shannon MA. Cytoskeletal networks in an epithelial sheet: Mechanical characterization using composite diaphragm inflation. In 2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology. 2005. p. 198-200. 1548425. (2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology). doi: 10.1109/MMB.2005.1548425

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Cytoskeletal networks in an epithelial sheet: Mechanical characterization using composite diaphragm inflation

Abstract

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