TY - JOUR
T1 - Cytoskeletal mechanics in adherent human airway smooth muscle cells
T2 - Probe specificity and scaling of protein-protein dynamics
AU - Puig-De-Morales, Marina
AU - Millet, Emil
AU - Fabry, Ben
AU - Navajas, Daniel
AU - Wang, Ning
AU - Butler, James P.
AU - Fredberg, Jeffrey J.
PY - 2004/9
Y1 - 2004/9
N2 - We probed elastic and loss moduli in the adherent human airway smooth muscle cell through a variety of receptor systems, each serving as a different molecular window on cytoskeletal dynamics. Coated magnetic microbeads were attached to the cell surface via coating-receptor binding. A panel of bead coatings was investigated: a peptide containing the sequence RGD, vitronectin, urokinase, activating antibody against β1-integrin, nonactivating antibody against β1-integrin, blocking antibody against β1-integrin, antibody against β1- integrin, and acetylated low-density lipoprotein. An oscillatory mechanical torque was applied to the bead, and resulting lateral displacements were measured at baseline, after actin disruption by cytochalasin D, or after contractile activation by histamine. As expected, mechanical moduli depended strongly on bead type and bead coating, differing at the extremes by as much as two orders of magnitude. In every case, however, elastic and loss moduli increased with frequency f as a weak power law, fx-1. Moreover, with few exceptions, data could be scaled such that elastic and frictional responses depended solely on the power law exponent x. Taken together, these data suggest that power law behavior represents a generic feature of underlying protein-protein dynamics.
AB - We probed elastic and loss moduli in the adherent human airway smooth muscle cell through a variety of receptor systems, each serving as a different molecular window on cytoskeletal dynamics. Coated magnetic microbeads were attached to the cell surface via coating-receptor binding. A panel of bead coatings was investigated: a peptide containing the sequence RGD, vitronectin, urokinase, activating antibody against β1-integrin, nonactivating antibody against β1-integrin, blocking antibody against β1-integrin, antibody against β1- integrin, and acetylated low-density lipoprotein. An oscillatory mechanical torque was applied to the bead, and resulting lateral displacements were measured at baseline, after actin disruption by cytochalasin D, or after contractile activation by histamine. As expected, mechanical moduli depended strongly on bead type and bead coating, differing at the extremes by as much as two orders of magnitude. In every case, however, elastic and loss moduli increased with frequency f as a weak power law, fx-1. Moreover, with few exceptions, data could be scaled such that elastic and frictional responses depended solely on the power law exponent x. Taken together, these data suggest that power law behavior represents a generic feature of underlying protein-protein dynamics.
KW - Actin
KW - Cytoskeleton
KW - Magnetic twisting cytometry
KW - Scale free
KW - Viscoelasticity
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U2 - 10.1152/ajpcell.00070.2004
DO - 10.1152/ajpcell.00070.2004
M3 - Article
C2 - 15175221
AN - SCOPUS:4143067956
SN - 0363-6143
VL - 287
SP - C643-C654
JO - American Journal of Physiology - Cell Physiology
JF - American Journal of Physiology - Cell Physiology
IS - 3 56-3
ER -