Cytoplasmic copper detoxification in Salmonella can contribute to SodC metalation but is dispensable during systemic infection

Luke A. Fenlon, James M. Slauch

Research output: Contribution to journalArticle

Abstract

Salmonella enterica serovar Typhimurium is a leading cause of foodborne disease worldwide. Severe infections result from the ability of S. Typhimurium to survive within host immune cells, despite being exposed to various host antimicrobial factors. SodCI, a copper-zinc-cofactored superoxide dismutase, is required to defend against phagocytic superoxide. SodCII, an additional periplasmic superoxide dismutase, although produced during infection, does not function in the host. Previous studies suggested that CueP, a periplasmic copper binding protein, facilitates acquisition of copper by SodCII. CopA and GolT, both inner membrane ATPases that pump copper from the cytoplasm to the periplasm, are a source of copper for CueP. Using in vitro SOD assays, we found that SodCI can also utilize CueP to acquire copper. However, both SodCI and SodCII have a significant fraction of activity independent of CueP and cytoplasmic copper export. We utilized a series of mouse competition assays to address the in vivo role of CueP-mediated SodC activation. A copA golT cueP triple mutant was equally as competitive as the wild type, suggesting that sufficient SodCI is active to defend against phagocytic superoxide independent of CueP and cytoplasmic copper export. We also confirmed that a strain containing a modified SodCII, which is capable of complementing a sodCI deletion, was fully virulent in a copA golT cueP background competed against the wild type. These competitions also address the potential impact of cytoplasmic copper toxicity within the phagosome. Our data suggest that Salmonella does not encounter inhibitory concentrations of copper during systemic infection.

Original languageEnglish (US)
Article numbere00437-17
JournalJournal of bacteriology
Volume199
Issue number24
DOIs
StatePublished - Jan 1 2017

Fingerprint

Salmonella
Copper
Infection
Superoxides
Superoxide Dismutase
Periplasmic Binding Proteins
Periplasm
Phagosomes
Foodborne Diseases
Salmonella enterica
Adenosine Triphosphatases
Zinc
Cytoplasm
Membranes

Keywords

  • Copper efflux
  • Salmonella
  • SodC

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Cytoplasmic copper detoxification in Salmonella can contribute to SodC metalation but is dispensable during systemic infection. / Fenlon, Luke A.; Slauch, James M.

In: Journal of bacteriology, Vol. 199, No. 24, e00437-17, 01.01.2017.

Research output: Contribution to journalArticle

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