TY - JOUR
T1 - Cytogenetic characterization of the Antarctic silverfish Pleuragramma antarctica (Boulenger 1902) through analysis of mitotic chromosomes from early larvae
AU - Ghigliotti, Laura
AU - Cheng, Chi Hing C.
AU - Ozouf-Costaz, Catherine
AU - Guidi-Rontani, Chantal
AU - Vacchi, Marino
AU - Federici, Sara
AU - Pisano, Eva
N1 - The work was carried out within the project IMAGES ( PdR 2013/AZ1.11 ) and POLICY ( PNRA16_00281 ) funded by the Italian National Programme for Antarctic Research (PNRA), and contributes to the SCAR Scientific Research Program AntERA (Antarctic Thresholds – Ecosystems Resilience and Adaptation). We thank Jean-Pierre Coutanceau for the technical support to the analysis at the Institute of Biology Paris Seine. CHCC acknowledges support from US NSF award OPP-1645087 .
PY - 2020/8
Y1 - 2020/8
N2 - This paper describes the cytogenetic features of the Antarctic silverfish Pleuragramma antarctica (Boulenger 1902), a keystone species of the Antarctic coastal marine ecosystem. Conventional cytogenetic analyses and physical mapping of repetitive DNA sequences were performed on metaphase plates obtained through direct chromosome preparation from P. antarctica early larvae. The Antarctic silverfish have a diploid number (2n) = 48, and a karyotype made up of a majority of two-armed chromosomes (karyotype formula36m/sm + 10st + 2a, fundamental number = 94). Major ribosomal gene repeats were detected on three chromosome pairs (20, 21, and 23), in correspondence of dim DAPI stained regions. Long Interspersed Nuclear Elements (LINEs) were abundant and wide spread over all chromosomes. Overall, the cytogenetic data presented herein are consistent with a long independent cytogenetic and evolutionary history for the species. The large number of two-armed chromosomes, indicative of highly-rearranged karyotype, coupled with a diploid number of 48, a presumed primitive character for this fish group, and the spread of the major ribosomal genes on three chromosome pairs, make the Antarctic silverfish distinct from all other notothenioid species.
AB - This paper describes the cytogenetic features of the Antarctic silverfish Pleuragramma antarctica (Boulenger 1902), a keystone species of the Antarctic coastal marine ecosystem. Conventional cytogenetic analyses and physical mapping of repetitive DNA sequences were performed on metaphase plates obtained through direct chromosome preparation from P. antarctica early larvae. The Antarctic silverfish have a diploid number (2n) = 48, and a karyotype made up of a majority of two-armed chromosomes (karyotype formula36m/sm + 10st + 2a, fundamental number = 94). Major ribosomal gene repeats were detected on three chromosome pairs (20, 21, and 23), in correspondence of dim DAPI stained regions. Long Interspersed Nuclear Elements (LINEs) were abundant and wide spread over all chromosomes. Overall, the cytogenetic data presented herein are consistent with a long independent cytogenetic and evolutionary history for the species. The large number of two-armed chromosomes, indicative of highly-rearranged karyotype, coupled with a diploid number of 48, a presumed primitive character for this fish group, and the spread of the major ribosomal genes on three chromosome pairs, make the Antarctic silverfish distinct from all other notothenioid species.
KW - Fluorescence in situ hybridization
KW - Karyotype
KW - LINEs
KW - Notothenioidei
KW - Ribosomal genes
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U2 - 10.1016/j.margen.2019.100737
DO - 10.1016/j.margen.2019.100737
M3 - Article
C2 - 31892467
AN - SCOPUS:85077164789
SN - 1874-7787
VL - 52
JO - Marine Genomics
JF - Marine Genomics
M1 - 100737
ER -