Cytochrome p450 gene clusters in Drosophila melanogaster

Boris C. Dunkov, Rosario Rodriguez-Arnaiz, Barry Robert Pittendrigh, Richard H. Ffrench-Constant, René Feyereisen

Research output: Contribution to journalArticle

Abstract

Twelve cytochrome P450 cDNA fragments were cloned from Drosophila melanogaster by reverse transcriptase/PCR (RT/PCR) using degenerate oligonucleotide primers. The corresponding genes belong to several subfamilies of the CYP4 and CYP9 P450 families. Only two of these genes, Cyp4d1 and Cyp4d2, have previously been described. In situ hybridization of each of the cDNA fragments showed two clusters of genes; one near the tip of the X chromosome and the other on the left arm of chromosome 2. Interestingly the latter cluster comprises widely divergent genes belonging both to the CYP9 and CYP4 families and also to the CYP6 family (Cyp6a2). Putative allelic variants of several of the genes were found in different insecticide-resistant and -susceptible strains (Hikone R, Haag 79 and Oregon R). The identification of these genes and alleles will allow us to clarify the involvement of P450s in xenobiotic metabolism and will facilitate a genetic analysis of P450 functions in insects.

Original languageEnglish (US)
Pages (from-to)290-297
Number of pages8
JournalMolecular and General Genetics
Volume251
Issue number3
DOIs
StatePublished - Jul 2 1996

Keywords

  • Cytochrome p450 genes
  • Drosophila melanogaster
  • Gene clusters
  • Insecticide resistance
  • Multigene family

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Cytochrome p450 gene clusters in Drosophila melanogaster'. Together they form a unique fingerprint.

  • Cite this

    Dunkov, B. C., Rodriguez-Arnaiz, R., Pittendrigh, B. R., Ffrench-Constant, R. H., & Feyereisen, R. (1996). Cytochrome p450 gene clusters in Drosophila melanogaster. Molecular and General Genetics, 251(3), 290-297. https://doi.org/10.1007/s004380050169