Cyclic changes in cAMP concentration and phosphodiesterase activity in a mammalian circadian clock studied in vitro

Rebecca A. Prosser; Martha U. Gillette

doi:10.1016/0006-8993(91)91396-I

Cyclic changes in cAMP concentration and phosphodiesterase activity in a mammalian circadian clock studied in vitro

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Abstract

The mammalian suprachiasmatic nuclei (SCN) contain a circadian pacemaker that continues to keep 24-h time when isolated in vitro. We are investigating the role of cAMP in the cellular mechanisms underlying SCN function. We have previously shown that increasing intracellular cAMP during the subjective day resets the SCN pacemaker in the in vitro rat brain slice preparation^8,20. We now report that the level of cAMP fluctuates within the rat SCN under constant conditions in vitro. The level of endogenous cAMP is high during late day and late night, and low during early night. These changes in cAMP concentration are accompanied by opposite changes in phosphodiesterase activity; we detected no significant change in adenylate cyclase activity. These results provide further support for the hypothesis that cAMP is involved in circadian function in the SCN.

Original language	English (US)
Pages (from-to)	185-192
Number of pages	8
Journal	Brain Research
Volume	568
Issue number	1-2
DOIs	https://doi.org/10.1016/0006-8993(91)91396-I
State	Published - Dec 24 1991

Keywords

Adenylate cyclase
Brain slice
Circadian rhythm
Cyclic adenosine monophosphate
Pacemaker
Phosphodiesterase
Suprachiasmatic nucleus

ASJC Scopus subject areas

Developmental Biology
Molecular Biology
Clinical Neurology
General Neuroscience

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10.1016/0006-8993(91)91396-I

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title = "Cyclic changes in cAMP concentration and phosphodiesterase activity in a mammalian circadian clock studied in vitro",

abstract = "The mammalian suprachiasmatic nuclei (SCN) contain a circadian pacemaker that continues to keep 24-h time when isolated in vitro. We are investigating the role of cAMP in the cellular mechanisms underlying SCN function. We have previously shown that increasing intracellular cAMP during the subjective day resets the SCN pacemaker in the in vitro rat brain slice preparation8,20. We now report that the level of cAMP fluctuates within the rat SCN under constant conditions in vitro. The level of endogenous cAMP is high during late day and late night, and low during early night. These changes in cAMP concentration are accompanied by opposite changes in phosphodiesterase activity; we detected no significant change in adenylate cyclase activity. These results provide further support for the hypothesis that cAMP is involved in circadian function in the SCN.",

keywords = "Adenylate cyclase, Brain slice, Circadian rhythm, Cyclic adenosine monophosphate, Pacemaker, Phosphodiesterase, Suprachiasmatic nucleus",

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AU - Gillette, Martha U.

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N2 - The mammalian suprachiasmatic nuclei (SCN) contain a circadian pacemaker that continues to keep 24-h time when isolated in vitro. We are investigating the role of cAMP in the cellular mechanisms underlying SCN function. We have previously shown that increasing intracellular cAMP during the subjective day resets the SCN pacemaker in the in vitro rat brain slice preparation8,20. We now report that the level of cAMP fluctuates within the rat SCN under constant conditions in vitro. The level of endogenous cAMP is high during late day and late night, and low during early night. These changes in cAMP concentration are accompanied by opposite changes in phosphodiesterase activity; we detected no significant change in adenylate cyclase activity. These results provide further support for the hypothesis that cAMP is involved in circadian function in the SCN.

AB - The mammalian suprachiasmatic nuclei (SCN) contain a circadian pacemaker that continues to keep 24-h time when isolated in vitro. We are investigating the role of cAMP in the cellular mechanisms underlying SCN function. We have previously shown that increasing intracellular cAMP during the subjective day resets the SCN pacemaker in the in vitro rat brain slice preparation8,20. We now report that the level of cAMP fluctuates within the rat SCN under constant conditions in vitro. The level of endogenous cAMP is high during late day and late night, and low during early night. These changes in cAMP concentration are accompanied by opposite changes in phosphodiesterase activity; we detected no significant change in adenylate cyclase activity. These results provide further support for the hypothesis that cAMP is involved in circadian function in the SCN.

KW - Adenylate cyclase

KW - Brain slice

KW - Circadian rhythm

KW - Cyclic adenosine monophosphate

KW - Pacemaker

KW - Phosphodiesterase

KW - Suprachiasmatic nucleus

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Cyclic changes in cAMP concentration and phosphodiesterase activity in a mammalian circadian clock studied in vitro

Abstract

Keywords

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