Cutting edge: Check your mice - A point mutation in the Ncr1 locus identified in CD45.1 congenic mice with consequences in mouse susceptibility to infection

Youngsoon Jang, Zachary J. Gerbec, Taejoon Won, Bongkum Choi, Amy Podsiad, Bethany B. Moore, Subramaniam Malarkannan, Yasmina Laouar

Research output: Contribution to journalArticlepeer-review

Abstract

B6.SJL-Ptprca Pepcb/Boy (CD45.1) mice have been used in hundreds of congenic competitive transplants, with the presumption that they differ from C57BL/6 mice only at the CD45 locus. In this study, we describe a point mutation in the natural cytotoxicity receptor 1 (Ncr1) locus fortuitously identified in the CD45.1 strain. This point mutation was mapped at the 40th nucleotide of the Ncr1 locus causing a single amino acid mutation from cysteine to arginine at position 14 from the start codon, resulting in loss of NCR1 expression. We found that these mice were more resistant to CMV due to a hyper innate IFN-γ response in the absence of NCR1. In contrast, loss of NCR1 increased susceptibility to influenza virus, a result that is consistent with the role of NCR1 in the recognition of influenza Ag, hemagglutinin. This work sheds light on potential confounding experimental interpretation when this congenic strain is used as a tool for tracking lymphocyte development.

Original languageEnglish (US)
Pages (from-to)1982-1987
Number of pages6
JournalJournal of Immunology
Volume200
Issue number6
DOIs
StatePublished - Mar 15 2018
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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