Crystallographic structures of two bisphosphonate:1-deoxyxylulose-5- phosphate reductoisomerase complexes

Shunsuke Yajima, Kodai Hara, John M. Sanders, Fenglin Yin, Kanju Ohsawa, Jochen Wiesner, Hassan Jomaa, Eric Oldfield

Research output: Contribution to journalArticlepeer-review


We have obtained the single-crystal X-ray crystallographic structures of the bisphosphonates [(1-isoquinolinylamino)methylene]-1,1-bisphosphonate and [[(5-chloro-2-pyridinyl)amino]methylene]-1,1-bisphosphonate, bound to the enzyme 1-deoxyxylulose-5-phosphate reductoisomerase (DXR, EC, also known as 2-C-methyl-d-erythritol-4-phosphate synthase), an important target for the development of antimalarial drugs. Our results indicate that both bisphosphonates bind into the fosmidomycin binding site. The aromatic groups are in a shallow hydrophobic pocket, and the phosphonate groups are involved in electrostatic interactions with Mg2+ or a cluster of carboxylic acid groups and lysine while the fosmidomycin phosphonate-binding site is occupied by a sulfate ion (as also observed in the DXR/NADP+ structure). The availability of these two new crystal structures opens up the possibility of the further development of bisphosphonates and related systems as DXR inhibitors and, potentially, as antiinfective agents.

Original languageEnglish (US)
Pages (from-to)10824-10825
Number of pages2
JournalJournal of the American Chemical Society
Issue number35
StatePublished - Sep 8 2004

ASJC Scopus subject areas

  • Catalysis
  • General Chemistry
  • Biochemistry
  • Colloid and Surface Chemistry


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