Crystal habit modification of protein by using microfluidic chip

M. Maeki, Ashtamurthy S Pawate, K. Watanabe, M. Tokeshi, Paul J A Kenis, M. Miyazaki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We demonstrate a novel method for controlling morphology and aggregation of protein crystals by focusing on the depth of microchannel. The microfluidic chip with normally closed valves was fabricated for crystallization experiments and in-situ X-ray analysis. Crystal habit of protein was dramatically changed by depth of crystallization chamber. We found the potential of microfluidic chip for controlling crystal habit as well as aggregation of protein crystals.

Original languageEnglish (US)
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages1083-1085
Number of pages3
ISBN (Electronic)9780979806476
StatePublished - Jan 1 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: Oct 26 2014Oct 30 2014

Publication series

Name18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period10/26/1410/30/14

Fingerprint

Microfluidics
Proteins
Crystals
Agglomeration
Crystallization
X ray analysis
Microchannels
Experiments

Keywords

  • Crystal habit
  • Microfluidics
  • Normally closed valve
  • Protein crystallization

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Maeki, M., Pawate, A. S., Watanabe, K., Tokeshi, M., Kenis, P. J. A., & Miyazaki, M. (2014). Crystal habit modification of protein by using microfluidic chip. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 1083-1085). (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014). Chemical and Biological Microsystems Society.

Crystal habit modification of protein by using microfluidic chip. / Maeki, M.; Pawate, Ashtamurthy S; Watanabe, K.; Tokeshi, M.; Kenis, Paul J A; Miyazaki, M.

18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. p. 1083-1085 (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Maeki, M, Pawate, AS, Watanabe, K, Tokeshi, M, Kenis, PJA & Miyazaki, M 2014, Crystal habit modification of protein by using microfluidic chip. in 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, Chemical and Biological Microsystems Society, pp. 1083-1085, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, San Antonio, United States, 10/26/14.
Maeki M, Pawate AS, Watanabe K, Tokeshi M, Kenis PJA, Miyazaki M. Crystal habit modification of protein by using microfluidic chip. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society. 2014. p. 1083-1085. (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).
Maeki, M. ; Pawate, Ashtamurthy S ; Watanabe, K. ; Tokeshi, M. ; Kenis, Paul J A ; Miyazaki, M. / Crystal habit modification of protein by using microfluidic chip. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. pp. 1083-1085 (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).
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